human Albumin Antibody | Affinity Biosciences

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Figure 1: Western blot analysis using human Albumin mouse mAb (lane 3, 4) and human IgG mouse mAb(lane 1, 2) against human serum (lane 1, 3) and plasma (lane 2, 4).

Product:	human Albumin Antibody
Catalog:	BF0544
Description:	Mouse monoclonal antibody to human Albumin
Application:	WB ELISA
Reactivity:	Human
Mol.Wt.:	69kDa; 69kD(Calculated).
Uniprot:	P02768
RRID:	AB_2833505

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Product Info

Source:

Mouse

Application:

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFB1480]

Specificity:

human Albumin antibody detects endogenous levels of total human Albumin.

RRID:

AB_2833505
Cite Format: Affinity Biosciences Cat# BF0544, RRID:AB_2833505.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

alb; ALBU_HUMAN; Albumin (32 AA); Albumin (AA 34); Albumin; Analbuminemia; Bisalbuminemia; Cell growth inhibiting protein 42; DKFZp779N1935; Dysalbuminemic hyperthyroxinemia; Growth inhibiting protein 20; HSA; Hyperthyroxinemia dysalbuminemic; PRO0883; PRO0903; PRO1341; Serum albumin;

Immunogens

Immunogen:

Purified recombinant fragment of human human Albumin expressed in E. Coli.

Uniprot:

P02768(ALBU_HUMAN) >>Visit HPA database.

Gene(ID):

ALB(213)

Expression:

P02768 ALBU_HUMAN:

Plasma.

Description:

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Sequence:

P02768 ALBU_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

PTMs - P02768 As Substrate

P02768

Site	PTM Type	Source
S14	Phosphorylation	Uniprot
Y17	Phosphorylation	Uniprot
S18	Phosphorylation	Uniprot
S29	Phosphorylation	Uniprot
K44	Acetylation	Uniprot
Y54	Phosphorylation	Uniprot
C58	S-Nitrosylation	Uniprot
K65	Acetylation	Uniprot
T71	Phosphorylation	Uniprot
K75	Acetylation	Uniprot
K75	Methylation	Uniprot
C77	S-Nitrosylation	Uniprot
S82	Phosphorylation	Uniprot
C86	S-Nitrosylation	Uniprot
S89	Phosphorylation	Uniprot
T92	Phosphorylation	Uniprot
C99	S-Nitrosylation	Uniprot
T107	Phosphorylation	Uniprot
Y108	Phosphorylation	Uniprot
C114	S-Nitrosylation	Uniprot
C115	S-Nitrosylation	Uniprot
K117	Acetylation	Uniprot
C125	S-Nitrosylation	Uniprot
K130	Acetylation	Uniprot
C148	S-Nitrosylation	Uniprot
K160	Acetylation	Uniprot
K161	Acetylation	Uniprot
Y162	Phosphorylation	Uniprot
Y164	Phosphorylation	Uniprot
R168	Methylation	Uniprot
Y172	Phosphorylation	Uniprot
Y174	Phosphorylation	Uniprot
K183	Acetylation	Uniprot
K186	Acetylation	Uniprot
T190	Phosphorylation	Uniprot
C192	S-Nitrosylation	Uniprot
C193	S-Nitrosylation	Uniprot
K198	Acetylation	Uniprot
C201	S-Nitrosylation	Uniprot
K214	Acetylation	Uniprot
S216	Phosphorylation	Uniprot
S217	Phosphorylation	Uniprot
K219	Acetylation	Uniprot
K223	Acetylation	Uniprot
K229	Acetylation	Uniprot
K229	Ubiquitination	Uniprot
S244	Phosphorylation	Uniprot
K249	Acetylation	Uniprot
S256	Phosphorylation	Uniprot
K257	Acetylation	Uniprot
T260	Phosphorylation	Uniprot
T263	Phosphorylation	Uniprot
K264	Acetylation	Uniprot
C269	S-Nitrosylation	Uniprot
C270	S-Nitrosylation	Uniprot
K286	Acetylation	Uniprot
Y287	Phosphorylation	Uniprot
C289	S-Nitrosylation	Uniprot
S294	Phosphorylation	Uniprot
S296	Phosphorylation	Uniprot
S297	Phosphorylation	Uniprot
K298	Acetylation	Uniprot
K298	Ubiquitination	Uniprot
K300	Acetylation	Uniprot
C303	S-Nitrosylation	Uniprot
K305	Acetylation	Uniprot
S311	Phosphorylation	Uniprot
C313	S-Nitrosylation	Uniprot
S328	Phosphorylation	Uniprot
S336	Phosphorylation	Uniprot
Y343	Phosphorylation	Uniprot
K347	Acetylation	Uniprot
Y365	Phosphorylation	Uniprot
S366	Phosphorylation	Uniprot
T376	Phosphorylation	Uniprot
Y377	Phosphorylation	Uniprot
T379	Phosphorylation	Uniprot
T380	Phosphorylation	Uniprot
C385	S-Nitrosylation	Uniprot
Y394	Phosphorylation	Uniprot
K396	Acetylation	Uniprot
K402	Acetylation	Uniprot
C416	S-Nitrosylation	Uniprot
Y425	Phosphorylation	Uniprot
K426	Acetylation	Uniprot
T436	Phosphorylation	Uniprot
K438	Acetylation	Uniprot
S443	Phosphorylation	Uniprot
T444	O-Glycosylation	Uniprot
T444	Phosphorylation	Uniprot
T446	Phosphorylation	Uniprot
S451	Phosphorylation	Uniprot
K456	Acetylation	Uniprot
K468	Acetylation	Uniprot
C472	S-Nitrosylation	Uniprot
Y476	Phosphorylation	Uniprot
C485	S-Nitrosylation	Uniprot
K490	Methylation	Uniprot
T498	Phosphorylation	Uniprot
K499	Acetylation	Uniprot
C500	S-Nitrosylation	Uniprot
T502	Phosphorylation	Uniprot
S504	Phosphorylation	Uniprot
C511	S-Nitrosylation	Uniprot
S513	Phosphorylation	Uniprot
T520	Phosphorylation	Uniprot
Y521	Phosphorylation	Uniprot
K524	Acetylation	Uniprot
C538	S-Nitrosylation	Uniprot
K549	Acetylation	Uniprot
T551	Phosphorylation	Uniprot
K558	Acetylation	Uniprot
K558	Methylation	Uniprot
K562	Acetylation	Uniprot
T564	Phosphorylation	Uniprot
T590	Phosphorylation	Uniprot
C591	S-Nitrosylation	Uniprot
K597	Acetylation	Uniprot
K598	Acetylation	Uniprot
S603	Phosphorylation	Uniprot

Research Backgrounds

P02768_ALBU_HUMAN

Function:

Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.

PTMs:

Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.

Glycated in diabetic patients.

Phosphorylated by FAM20C in the extracellular medium.

Acetylated on Lys-223 by acetylsalicylic acid.

Subcellular Location:

Secreted.

Tissue Specificity:

Plasma.

Subunit Structure:

Interacts with FCGRT; this interaction regulates ALB homeostasis.

Family&Domains:

Belongs to the ALB/AFP/VDB family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

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For Research Use Only.
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human Albumin Antibody - #BF0544

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