SOX2 Antibody - #BF0481

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Product: SOX2 Antibody
Catalog: BF0481
Description: Mouse monoclonal antibody to SOX2
Application: WB IHC IF/ICC ELISA
Reactivity: Human
Mol.Wt.: 34.3kDa; 34kD(Calculated).
Uniprot: P48431
RRID: AB_2833503

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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
ELISA 1:10000, WB 1:500-1:2000, IHC 1：200, IF 1：200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFB1478]
Specificity:
SOX2 antibody detects endogenous levels of total SOX2.
RRID:
AB_2833503
Cite Format: Affinity Biosciences Cat# BF0481, RRID:AB_2833503.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; SOX 2; Sox2; SOX2_HUMAN; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; Transcription factor SOX 2; Transcription factor SOX-2; ysb;

Immunogens

Immunogen:

Purified recombinant fragment of human SOX2 expressed in E. Coli.

Uniprot:

P48431(SOX2_HUMAN) >>Visit HPA database.

Gene(ID):
SOX2(6657)
Description:
SOX2: SRY (sex determining region Y)-box 2. Entrez Protein NP_003097. It is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Sequence:
MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM

PTMs - P48431 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
K73 Acetylation
T116 Phosphorylation
T118 Phosphorylation P31749 (AKT1)
K121 Ubiquitination
Y125 Phosphorylation
T126 Phosphorylation
S138 Phosphorylation
S220 Phosphorylation
K245 Sumoylation
S246 Phosphorylation
S249 Phosphorylation
S250 Phosphorylation
S251 Phosphorylation

Research Backgrounds

Function:

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

PTMs:

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ZSCAN10 (By similarity). Interacts with SOX3 and FGFR1 (By similarity). Interacts with GLIS1. Interacts with POU5F1; binds synergistically with POU5F1 to DNA (By similarity).

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

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