AFfirm™ IST1 Mouse Monoclonal Antibody - #BF9506

Product: IST1 Mouse Monoclonal Antibody
Catalog: BF9506
Description: Mouse monoclonal antibody to IST1
Application: WB
Reactivity: Human
Prediction: Mouse, Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40 kDa; 40kD(Calculated).
Uniprot: P53990

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9506]
Specificity:
IST1 Mouse Monoclonal Antibody detects endogenous levels of total IST1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hIST1; Increased sodium tolerance 1; Increased sodium tolerance 1 homolog; IST1 homolog; IST1_HUMAN; KIAA0174; MAPK activating protein PM28; MGC117220; MGC128433; Putative MAPK-activating protein PM28;

Immunogens

Immunogen:

A synthesized peptide derived from human IST1, corresponding to a region within the internal amino acids.

Uniprot:

P53990(IST1_HUMAN) >>Visit HPA database.

Gene(ID):
IST1(9798)
Sequence:
MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT

Research Backgrounds

Function:

ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.

Subcellular Location:

Cytoplasmic vesicle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody. Nucleus envelope.
Note: Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the nuclear envelope during late anaphase (PubMed:26040712).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the IST1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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For Research Use Only.
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