AFfirm™ ASF1B Mouse Monoclonal Antibody - #BF9430

Product: ASF1B Mouse Monoclonal Antibody
Catalog: BF9430
Description: Mouse monoclonal antibody to ASF1B
Application: WB
Reactivity: Human
Prediction: Mouse, Bovine, Sheep, Rabbit, Xenopus
Mol.Wt.: 26kDa; 22kD(Calculated).
Uniprot: Q9NVP2

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm9430]
Specificity:
ASF1B Mouse Monoclonal Antibody detects endogenous levels of total ASF1B.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Anti silencing function 1B; Anti silencing function 1B histone chaperone; Anti-silencing function protein 1 homolog B; ASF1 anti silencing function 1 homolog B (S. cerevisiae); ASF1 anti silencing function 1 homolog B; ASF1B; ASF1B_HUMAN; CCG1 interacting factor A II; CCG1-interacting factor A-II; CIA-II; hAsf1; hAsf1b; hCIA-II; Histone chaperone ASF1B;

Immunogens

Immunogen:
Uniprot:

Q9NVP2(ASF1B_HUMAN) >>Visit HPA database.

Gene(ID):
ASF1B(55723)
Expression:
Q9NVP2 ASF1B_HUMAN:

Highly expressed in testis and at lower levels in colon, small intestine and thymus.

Sequence:
MAKVSVLNVAVLENPSPFHSPFRFEISFECSEALADDLEWKIIYVGSAESEEFDQILDSVLVGPVPAGRHMFVFQADAPNPSLIPETDAVGVTVVLITCTYHGQEFIRVGYYVNNEYLNPELRENPPMKPDFSQLQRNILASNPRVTRFHINWDNNMDRLEAIETQDPSLGCGLPLNCTPIKGLGLPGCIPGLLPENSMDCI

PTMs - Q9NVP2 As Substrate

Site PTM Type Enzyme
K3 Ubiquitination
Y111 Phosphorylation
Y117 Phosphorylation
K129 Sumoylation
K129 Ubiquitination
S142 Phosphorylation
T179 Phosphorylation
S198 Phosphorylation Q86UE8 (TLK2)

Research Backgrounds

Function:

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.

PTMs:

Phosphorylated by TLK1 and TLK2.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and at lower levels in colon, small intestine and thymus.

Subunit Structure:

Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1A, IPO4 and histones H3.1 and H4. Interacts with CREBBP.

Family&Domains:

Belongs to the ASF1 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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