PGP9.5 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	PGP9.5 Mouse Monoclonal Antibody
Catalog:	BF8987
Description:	Mouse monoclonal antibody to PGP9.5
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	25 kDa; 25kD(Calculated).
Uniprot:	P09936

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8987]

Specificity:

PGP9.5 Mouse Monoclonal Antibody detects endogenous levels of total PGP9.5.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Epididymis luminal protein 117; Epididymis secretory protein Li 53; HEL 117; HEL S 53; NDGOA; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; Park 5; PARK5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase; Ubiquitin C terminal hydrolase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH-L1; UCHL1; UCHL1_HUMAN;

Immunogens

Immunogen:

Uniprot:

P09936(UCHL1_HUMAN) >>Visit HPA database.

Gene(ID):

UCHL1(7345)

Expression:

P09936 UCHL1_HUMAN:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Description:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes.

Sequence:

P09936 UCHL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

PTMs - P09936 As Substrate

P09936

Site	PTM Type	Source
K4	Acetylation	Uniprot
K4	Ubiquitination	Uniprot
K15	Ubiquitination	Uniprot
K64	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
K71	Ubiquitination	Uniprot
K78	Ubiquitination	Uniprot
K83	Ubiquitination	Uniprot
K105	Ubiquitination	Uniprot
K115	Ubiquitination	Uniprot
S119	Phosphorylation	Uniprot
K123	Ubiquitination	Uniprot
C132	S-Nitrosylation	Uniprot
K135	Ubiquitination	Uniprot
C152	S-Nitrosylation	Uniprot
K157	Ubiquitination	Uniprot
S188	Phosphorylation	Uniprot
S189	Phosphorylation	Uniprot
T192	Phosphorylation	Uniprot
K195	Acetylation	Uniprot
K195	Ubiquitination	Uniprot
C220	S-Nitrosylation	Uniprot
K221	Ubiquitination	Uniprot

Research Backgrounds

P09936_UCHL1_HUMAN

Function:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity.

PTMs:

O-glycosylated.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum membrane>Lipid-anchor.
Note: About 30% of total UCHL1 is associated with membranes in brain.

Tissue Specificity:

Subunit Structure:

Monomer. Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.

Family&Domains:

Belongs to the peptidase C12 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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AFfirm™ PGP9.5 Mouse Monoclonal Antibody - #BF8987