AFfirm™ Cytokeratin 16 Mouse Monoclonal Antibody - #BF8984

Product: Cytokeratin 16 Mouse Monoclonal Antibody
Catalog: BF8984
Description: Mouse monoclonal antibody to Cytokeratin 16
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Horse, Rabbit
Mol.Wt.: 50~60kD; 51kD(Calculated).
Uniprot: P08779

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8984]
Specificity:
Cytokeratin 16 Mouse Monoclonal Antibody detects endogenous levels of total Cytokeratin 16.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 16; CK-16; CK16; Cytokeratin-16; Cytokeratin16; FNEPPK; Focal non epidermolytic palmoplantar keratoderma; K 16; K16; K1C16_HUMAN; K1CP; Keratin 1 type I; Keratin 16; Keratin; Keratin type I cytoskeletal 16; Keratin-16; Keratin16; KRT 16; Krt16; KRT16A; NEPPK; PC1; type I cytoskeletal 16;

Immunogens

Immunogen:
Uniprot:

P08779(K1C16_HUMAN) >>Visit HPA database.

Gene(ID):
KRT16(3868)
Expression:
P08779 K1C16_HUMAN:

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

Description:
Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Sequence:
MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

PTMs - P08779 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S10 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation
K15 Methylation
S17 Phosphorylation
S28 Phosphorylation
S32 Phosphorylation
S33 Phosphorylation
S39 Phosphorylation
S44 Phosphorylation
T45 Phosphorylation
Y46 Phosphorylation
S51 Phosphorylation
S54 Phosphorylation
S130 Phosphorylation
Y131 Phosphorylation
K134 Acetylation
K134 Ubiquitination
Y153 Phosphorylation
K162 Acetylation
S165 Phosphorylation
Y167 Phosphorylation
K169 Acetylation
T204 Phosphorylation
Y206 Phosphorylation
T215 Phosphorylation
T235 Phosphorylation
Y250 Phosphorylation
Y293 Phosphorylation
K311 Ubiquitination
K317 Ubiquitination
S321 Phosphorylation
S329 Phosphorylation
S360 Phosphorylation
K365 Ubiquitination
K407 Ubiquitination
T416 Phosphorylation
Y417 Phosphorylation
Y438 Phosphorylation
S449 Phosphorylation
S450 Phosphorylation
S451 Phosphorylation
S452 Phosphorylation
K460 Methylation
S463 Phosphorylation
S464 Phosphorylation
S468 Phosphorylation
S472 Phosphorylation
S473 Phosphorylation

Research Backgrounds

Function:

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Tissue Specificity:

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

Subunit Structure:

Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers (KRT6A or KRT6B) (By similarity). Interacts with TCHP. Interacts with TRADD (By similarity).

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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