P Glycoprotein 1（MDR1） Mouse Monoclonal Antibody

Product:	P Glycoprotein 1（MDR1） Mouse Monoclonal Antibody
Catalog:	BF8972
Description:	Mouse monoclonal antibody to P Glycoprotein 1（MDR1）
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	141 kDa; 141kD(Calculated).
Uniprot:	P08183

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8972]

Specificity:

P Glycoprotein 1（MDR1） Mouse Monoclonal Antibody detects endogenous levels of total P Glycoprotein 1（MDR1）.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ABC20; ABCB1; ATP binding cassette, sub family B (MDR/TAP), member 1; ATP-binding cassette sub-family B member 1; CD243; CLCS; Colchicin sensitivity; Doxorubicin resistance; GP170; MDR1; MDR1_HUMAN; Multidrug resistance 1; Multidrug resistance protein 1; P glycoprotein 1; P gp; P-glycoprotein 1; PGY1;

Immunogens

Immunogen:

Uniprot:

P08183(MDR1_HUMAN) >>Visit HPA database.

Gene(ID):

ABCB1(5243)

Expression:

P08183 MDR1_HUMAN:

Expressed in liver, kidney, small intestine and brain.

Description:

A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon.

Sequence:

P08183 MDR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

PTMs - P08183 As Substrate

P08183

Site	PTM Type	Enzyme	Source
S23	Phosphorylation		Uniprot
T263	Phosphorylation		Uniprot
K271	Ubiquitination		Uniprot
K279	Ubiquitination		Uniprot
K367	Ubiquitination		Uniprot
K372	Ubiquitination		Uniprot
K380	Ubiquitination		Uniprot
K384	Acetylation		Uniprot
K389	Ubiquitination		Uniprot
K405	Sumoylation		Uniprot
K408	Sumoylation		Uniprot
K411	Ubiquitination		Uniprot
T422	Phosphorylation		Uniprot
T496	Phosphorylation		Uniprot
K515	Ubiquitination		Uniprot
K519	Ubiquitination		Uniprot
T522	Phosphorylation		Uniprot
K536	Ubiquitination		Uniprot
K617	Ubiquitination		Uniprot
K619	Ubiquitination		Uniprot
S644	Phosphorylation		Uniprot
S658	Phosphorylation		Uniprot
S660	Phosphorylation		Uniprot
S661	Phosphorylation	P17252 (PRKCA)	Uniprot
S667	Phosphorylation	P17612 (PRKACA) , P17252 (PRKCA)	Uniprot
S671	Phosphorylation	P17252 (PRKCA) , P17612 (PRKACA)	Uniprot
S675	Phosphorylation		Uniprot
S683	Phosphorylation	P17612 (PRKACA)	Uniprot
T684	Phosphorylation		Uniprot
S696	Phosphorylation		Uniprot
K826	Ubiquitination		Uniprot
K915	Ubiquitination		Uniprot
T1015	Phosphorylation		Uniprot
T1023	Phosphorylation		Uniprot
T1030	Phosphorylation		Uniprot
K1062	Ubiquitination		Uniprot
Y1087	Phosphorylation		Uniprot
K1093	Ubiquitination		Uniprot
K1099	Ubiquitination		Uniprot
K1102	Methylation		Uniprot
K1172	Ubiquitination		Uniprot
K1181	Ubiquitination		Uniprot
K1212	Ubiquitination		Uniprot
Y1267	Phosphorylation		Uniprot
S1269	Phosphorylation		Uniprot

Research Backgrounds

P08183_MDR1_HUMAN

Function:

Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Apical cell membrane.

Tissue Specificity:

Expressed in liver, kidney, small intestine and brain.

Subunit Structure:

Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

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AFfirm™ P Glycoprotein 1（MDR1） Mouse Monoclonal Antibody - #BF8972