SOD1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	SOD1 Mouse Monoclonal Antibody
Catalog:	BF8966
Description:	Mouse monoclonal antibody to SOD1
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	20kD; 16kD(Calculated).
Uniprot:	P00441

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8966]

Specificity:

SOD1 Mouse Monoclonal Antibody detects endogenous levels of total SOD1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALS; ALS1; Amyotrophic lateral sclerosis 1 adult; Cu/Zn SOD; Cu/Zn superoxide dismutase; Epididymis secretory protein Li 44; HEL S 44; Homodimer; hSod1; Indophenoloxidase A; IPOA; Mn superoxide dismutase; SOD; SOD soluble; SOD1; SOD2; SODC; SODC_HUMAN; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; Superoxide dismutase 1 soluble; Superoxide dismutase Cu Zn; Superoxide dismutase cystolic;

Immunogens

Immunogen:

Uniprot:

P00441(SODC_HUMAN) >>Visit HPA database.

Gene(ID):

SOD1(6647)

Description:

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Sequence:

P00441 SODC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

PTMs - P00441 As Substrate

P00441

Site	PTM Type	Source
A2	Acetylation	Uniprot
T3	Phosphorylation	Uniprot
K4	Ubiquitination	Uniprot
C7	S-Nitrosylation	Uniprot
K10	Acetylation	Uniprot
K10	Ubiquitination	Uniprot
K24	Acetylation	Uniprot
K24	Ubiquitination	Uniprot
S26	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
S35	Phosphorylation	Uniprot
K37	Ubiquitination	Uniprot
T40	Phosphorylation	Uniprot
T59	Phosphorylation	Uniprot
S60	Phosphorylation	Uniprot
S69	Phosphorylation	Uniprot
K71	Acetylation	Uniprot
K71	Ubiquitination	Uniprot
K76	Sumoylation	Uniprot
K76	Ubiquitination	Uniprot
R80	Methylation	Uniprot
T89	Phosphorylation	Uniprot
K92	Ubiquitination	Uniprot
S99	Phosphorylation	Uniprot
S103	Phosphorylation	Uniprot
S106	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
K123	Acetylation	Uniprot
K123	Ubiquitination	Uniprot
K129	Ubiquitination	Uniprot
K137	Ubiquitination	Uniprot
R144	Methylation	Uniprot
C147	S-Nitrosylation	Uniprot

Research Backgrounds

P00441_SODC_HUMAN

Function:

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

PTMs:

Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.

The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.

Palmitoylation helps nuclear targeting and decreases catalytic activity.

Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.

Subcellular Location:

Cytoplasm. Mitochondrion. Nucleus.
Note: Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.

Subunit Structure:

Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not.

Family&Domains:

Belongs to the Cu-Zn superoxide dismutase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

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AFfirm™ SOD1 Mouse Monoclonal Antibody - #BF8966