Casein Kinase 1 alpha Mouse Monoclonal Antibody

Product:	Casein Kinase 1 alpha Mouse Monoclonal Antibody
Catalog:	BF8962
Description:	Mouse monoclonal antibody to Casein Kinase 1 alpha
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat
Mol.Wt.:	39kD(Calculated).
Uniprot:	P48729

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8962]

Specificity:

Casein Kinase 1 alpha Mouse Monoclonal Antibody detects endogenous levels of total Casein Kinase 1 alpha.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975;

Immunogens

Immunogen:

A synthesized peptide derived from human Casein Kinase 1 alpha.

Uniprot:

P48729(KC1A_HUMAN) >>Visit HPA database.

Gene(ID):

CSNK1A1(1452)

Sequence:

P48729 KC1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF

PTMs - P48729 As Substrate

P48729

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S3	Phosphorylation		Uniprot
S4	Phosphorylation		Uniprot
K8	Acetylation		Uniprot
K8	Ubiquitination		Uniprot
K16	Acetylation		Uniprot
K16	Ubiquitination		Uniprot
Y17	Phosphorylation		Uniprot
Y32	Phosphorylation		Uniprot
K51	Ubiquitination		Uniprot
Y59	Phosphorylation		Uniprot
K62	Ubiquitination		Uniprot
K65	Ubiquitination		Uniprot
Y85	Phosphorylation		Uniprot
S105	Phosphorylation		Uniprot
K138	Ubiquitination		Uniprot
K152	Ubiquitination		Uniprot
K162	Ubiquitination		Uniprot
K179	Ubiquitination		Uniprot
T184	Phosphorylation		Uniprot
S199	Phosphorylation		Uniprot
S218	Phosphorylation	P17612 (PRKACA)	Uniprot
K225	Ubiquitination		Uniprot
K229	Ubiquitination		Uniprot
K240	Ubiquitination		Uniprot
S242	Phosphorylation	P17612 (PRKACA)	Uniprot
Y274	Phosphorylation		Uniprot
T287	Phosphorylation		Uniprot
Y292	Phosphorylation		Uniprot
Y294	Phosphorylation		Uniprot
K302	Ubiquitination		Uniprot
K304	Ubiquitination		Uniprot
S311	Phosphorylation		Uniprot
S313	Phosphorylation		Uniprot
T321	Phosphorylation		Uniprot
T323	Phosphorylation		Uniprot
K325	Ubiquitination		Uniprot
T327	Phosphorylation		Uniprot
S332	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot

PTMs - P48729 As Enzyme

P48729

Substrate	Site	Source
O15151 (MDM4)	S289	Uniprot
O43524 (FOXO3)	S318	Uniprot
O43524 (FOXO3)	S321	Uniprot
O60260 (PRKN)	S101	Uniprot
O60260 (PRKN)	S378	Uniprot
O60346 (PHLPP1)	S1359	Uniprot
O60346 (PHLPP1)	T1363	Uniprot
O60346 (PHLPP1)	S1379	Uniprot
O60346 (PHLPP1)	S1381	Uniprot
O75581 (LRP6)	T1493	Uniprot
O94916 (NFAT5)	S158	Uniprot
P01106 (MYC)	S252	Uniprot
P02730 (SLC4A1)	T42	Uniprot
P02730 (SLC4A1)	S303	Uniprot
P04637-1 (TP53)	S6	Uniprot
P04637-1 (TP53)	S9	Uniprot
P04637 (TP53)	T18	Uniprot
P04637 (TP53)	S20	Uniprot
P07910-2 (HNRNPC)	S240	Uniprot
P07910-1 (HNRNPC)	S253	Uniprot
P07910 (HNRNPC)	S260	Uniprot
P07910 (HNRNPC)	S299	Uniprot
P11308 (ERG)	S44	Uniprot
P11308 (ERG)	S45	Uniprot
P11308 (ERG)	S46	Uniprot
P12830 (CDH1)	S844	Uniprot
P16220 (CREB1)	S108	Uniprot
P16220 (CREB1)	S111	Uniprot
P16220 (CREB1)	S114	Uniprot
P16220 (CREB1)	S156	Uniprot
P17181 (IFNAR1)	S535	Uniprot
P17931 (LGALS3)	S6	Uniprot
P17931 (LGALS3)	S12	Uniprot
P18846 (ATF1)	S36	Uniprot
P18846 (ATF1)	S41	Uniprot
P18846 (ATF1)	S47	Uniprot
P18846 (ATF1)	S50	Uniprot
P18846 (ATF1)	S51	Uniprot
P25054 (APC)	S1504	Uniprot
P25054 (APC)	S1505	Uniprot
P25054 (APC)	S1507	Uniprot
P25054 (APC)	S1510	Uniprot
P27348 (YWHAQ)	S232	Uniprot
P30304 (CDC25A)	S79	Uniprot
P30304 (CDC25A)	S82	Uniprot
P35222 (CTNNB1)	S45	Uniprot
P37840 (SNCA)	S87	Uniprot
P37840 (SNCA)	S129	Uniprot
P40337 (VHL)	S72	Uniprot
P42892 (ECE1)	S34	Uniprot
P42892 (ECE1)	S36	Uniprot
P49810-1 (PSEN2)	S7	Uniprot
P49810-1 (PSEN2)	S9	Uniprot
P49810-3 (PSEN2)	S19	Uniprot
P55316 (FOXG1)	S19	Uniprot
P55957-1 (BID)	T59	Uniprot
P55957 (BID)	S64	Uniprot
P56537 (EIF6)	S174	Uniprot
P56537 (EIF6)	S175	Uniprot
P56817 (BACE1)	S498	Uniprot
P62745 (RHOB)	S185	Uniprot
P63104-1 (YWHAZ)	T232	Uniprot
Q00535 (CDK5)	S159	Uniprot
Q03060-12 (CREM)	S36	Uniprot
Q03060-30 (CREM)	S50	Uniprot
Q03060-32 (CREM)	S66	Uniprot
Q03060-16 (CREM)	S110	Uniprot
Q04206 (RELA)	S316	Uniprot
Q05940-1 (SLC18A2)	S511	Uniprot
Q12778 (FOXO1)	S322	Uniprot
Q12778 (FOXO1)	S325	Uniprot
Q12968-2 (NFATC3)	T204	Uniprot
Q12968-1 (NFATC3)	S207	Uniprot
Q12968-2 (NFATC3)	T210	Uniprot
Q12968-1 (NFATC3)	S211	Uniprot
Q12968-3 (NFATC3)	S215	Uniprot
Q13144 (EIF2B5)	S51	Uniprot
Q13144 (EIF2B5)	S466	Uniprot
Q13144 (EIF2B5)	S469	Uniprot
Q13148 (TARDBP)	S2	Uniprot
Q13148 (TARDBP)	Y4	Uniprot
Q13148 (TARDBP)	T25	Uniprot
Q13148 (TARDBP)	T88	Uniprot
Q13148 (TARDBP)	S91	Uniprot
Q13148 (TARDBP)	S92	Uniprot
Q13148 (TARDBP)	T116	Uniprot
Q13148 (TARDBP)	S183	Uniprot
Q13148 (TARDBP)	S242	Uniprot
Q13148 (TARDBP)	S254	Uniprot
Q13148 (TARDBP)	S273	Uniprot
Q13148 (TARDBP)	S292	Uniprot
Q13148 (TARDBP)	S305	Uniprot
Q13148 (TARDBP)	S342	Uniprot
Q13148 (TARDBP)	S347	Uniprot
Q13148 (TARDBP)	S350	Uniprot
Q13148 (TARDBP)	S369	Uniprot
Q13148 (TARDBP)	S375	Uniprot
Q13148 (TARDBP)	S377	Uniprot
Q13148 (TARDBP)	S379	Uniprot
Q13148 (TARDBP)	S387	Uniprot
Q13148 (TARDBP)	S389	Uniprot
Q13148 (TARDBP)	S393	Uniprot
Q13148 (TARDBP)	S395	Uniprot
Q13148 (TARDBP)	S403	Uniprot
Q13148 (TARDBP)	S404	Uniprot
Q13148 (TARDBP)	S407	Uniprot
Q13148 (TARDBP)	S409	Uniprot
Q13148 (TARDBP)	S410	Uniprot
Q13158 (FADD)	S194	Uniprot
Q6IE81 (JADE1)	S18	Uniprot
Q6IE81 (JADE1)	S20	Uniprot
Q8TB45 (DEPTOR)	T149	Uniprot
Q8TB45 (DEPTOR)	S263	Uniprot
Q8TB45 (DEPTOR)	S286	Uniprot
Q8TB45 (DEPTOR)	S287	Uniprot
Q8TB45 (DEPTOR)	S291	Uniprot
Q9BXL7 (CARD11)	S615	Uniprot
Q9H6E5 (TUT1)	S6	Uniprot
Q9NRF8 (CTPS2)	S568	Uniprot
Q9Y2W7 (KCNIP3)	S63	Uniprot
Q9Y4G8 (RAPGEF2)	S1244	Uniprot
Q9Y4G8 (RAPGEF2)	S1248	Uniprot

Research Backgrounds

P48729_KC1A_HUMAN

Function:

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Nucleus speckle. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688).

Subunit Structure:

Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation. Interacts with FAM83H; recruits CSNK1A1 to keratin filaments.

Family&Domains:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ Casein Kinase 1 alpha Mouse Monoclonal Antibody - #BF8962