AFfirm™ NF2 / Merlin Mouse Monoclonal Antibody - #BF8948
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
ACN; BANF; Bilateral acoustic neuroma; MERL_HUMAN; Merlin; Moesin ezrin radixin like protein; Moesin ezrin radizin like; Moesin-ezrin-radixin-like protein; Neurofibromatosis 2; Neurofibromatosis type 2; Neurofibromatosis2; Neurofibromin 2; Neurofibromin-2; Neurofibromin2; NF 2; Nf2; SCH; Schwannomerlin; Schwannomin;
Immunogens
Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
- P35240 MERL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKIYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFAIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL
PTMs - P35240 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | P31749 (AKT1) , P17612 (PRKACA) | Uniprot |
| S13 | Phosphorylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| K76 | Sumoylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| T230 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K253 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K279 | Acetylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K322 | Ubiquitination | Uniprot | |
| R351 | Methylation | Uniprot | |
| R353 | Methylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| T419 | Phosphorylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Q13153 (PAK1) , Q13177 (PAK2) , P17612 (PRKACA) | Uniprot |
| K533 | Ubiquitination | Uniprot | |
| K550 | Sumoylation | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| R568 | Methylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| T576 | Phosphorylation | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| T581 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| S587 | Phosphorylation | Uniprot |
Research Backgrounds
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. The dephosphorylation of Ser-518 favors the interaction with NOP53.
Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.
Cell projection>Filopodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus.
Note: In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.
Cytoplasm>Perinuclear region. Cytoplasmic granule.
Note: Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia.
Cytoplasm>Perinuclear region. Cytoplasmic granule.
Note: Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia.
Nucleus. Cell projection>Filopodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Perinuclear region. Cytoplasmic granule. Cytoplasm>Cytoskeleton.
Note: In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia.
Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Interacts with SLC9A3R1, HGS and AGAP2. Interacts with LAYN (By similarity). Interacts with SGSM3. Interacts (via FERM domain) with MPP1. Interacts with WWC1. Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1. Interacts (via FERM domain) with NOP53; the interaction is direct. Interacts with SCHIP1; the interaction is direct.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
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