Bcr Mouse Monoclonal Antibody | Affinity Biosciences

Product:	Bcr Mouse Monoclonal Antibody
Catalog:	BF8945
Description:	Mouse monoclonal antibody to Bcr
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Zebrafish, Chicken
Mol.Wt.:	160kDa; 143kD(Calculated).
Uniprot:	P11274

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8945]

Specificity:

Bcr Mouse Monoclonal Antibody detects endogenous levels of total Bcr.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALL; bcr; BCR/ABL FUSION GENE, INCLUDED; BCR/FGFR1 chimera protein; BCR/FGFR1 FUSION GENE, INCLUDED; BCR/PDGFRA FUSION GENE, INCLUDED; BCR_HUMAN; BCR1; Breakpoint cluster region; Breakpoint cluster region protein; CML; D22S11; D22S662; FGFR1/BCR chimera protein; PHL; Renal carcinoma antigen NY-REN-26;

Immunogens

Immunogen:

Uniprot:

P11274(BCR_HUMAN) >>Visit HPA database.

Gene(ID):

BCR(613)

Description:

Bcr is a serine/threonine and GTPase-activating protein (GAP). breakpoint cluster region protein. GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them.

Sequence:

P11274 BCR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVVSEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDDSPSSSPHLSSKGRGSRDALVSGALESTKASELDLEKGLEMRKWVLSGILASEETYLSHLEALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQRVGDLFQKLASQLGVYRAFVDNYGVAMEMAEKCCQANAQFAEISENLRARSNKDAKDPTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTPASHPDHPLLQDALRISQNFLSSINEEITPRRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDCKWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKNDIQREKRANKGSKATERLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFRSFSLTSVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLYGFLNVIVHSATGFKQSSNLYCTLEVDSFGYFVNKAKTRVYRDTAEPNWNEEFEIELEGSQTLRILCYEKCYNKTKIPKEDGESTDRLMGKGQVQLDPQALQDRDWQRTVIAMNGIEVKLSVKFNSREFSLKRMPSRKQTGVFGVKIAVVTKRERSKVPYIVRQCVEEIERRGMEEVGIYRVSGVATDIQALKAAFDVNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDEFYPNFAEGIALSDPVAKESCMLNLLLSLPEANLLTFLFLLDHLKRVAEKEAVNKMSLHNLATVFGPTLLRPSEKESKLPANPSQPITMTDSWSLEVMSQVQVLLYFLQLEAIPAPDSKRQSILFSTEV

PTMs - P11274 As Substrate

P11274

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S18	Phosphorylation		Uniprot
Y58	Phosphorylation		Uniprot
S88	Phosphorylation		Uniprot
S122	Phosphorylation		Uniprot
T130	Phosphorylation		Uniprot
S139	Phosphorylation		Uniprot
Y177	Phosphorylation	P06241 (FYN) , A9UF07 (BCR/ABL fusion) , P07332 (FES) , P08631 (HCK) , P00519 (ABL1) , A0A173G4P4 (Abl fusion) , P07948 (LYN)	Uniprot
K194	Acetylation		Uniprot
S201	Phosphorylation		Uniprot
S202	Phosphorylation		Uniprot
S205	Phosphorylation		Uniprot
K213	Acetylation		Uniprot
K214	Ubiquitination		Uniprot
S215	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
S222	Phosphorylation		Uniprot
S227	Phosphorylation		Uniprot
Y231	Phosphorylation		Uniprot
S235	Phosphorylation		Uniprot
S236	Phosphorylation		Uniprot
S239	Phosphorylation		Uniprot
C240	S-Nitrosylation		Uniprot
Y246	Phosphorylation	P07332 (FES)	Uniprot
S267	Phosphorylation		Uniprot
Y276	Phosphorylation	P07332 (FES)	Uniprot
Y279	Phosphorylation	P07332 (FES)	Uniprot
S281	Phosphorylation		Uniprot
Y283	Phosphorylation	A9UF07 (BCR/ABL fusion) , P07332 (FES)	Uniprot
S299	Phosphorylation		Uniprot
S301	Phosphorylation		Uniprot
T302	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
T310	Phosphorylation		Uniprot
S317	Phosphorylation		Uniprot
S320	Phosphorylation		Uniprot
Y328	Phosphorylation	A9UF07 (BCR/ABL fusion)	Uniprot
T329	Phosphorylation		Uniprot
S340	Phosphorylation		Uniprot
S351	Phosphorylation		Uniprot
S354	Phosphorylation		Uniprot
S356	Phosphorylation		Uniprot
T358	Phosphorylation		Uniprot
T359	Phosphorylation		Uniprot
Y360	Phosphorylation	A9UF07 (BCR/ABL fusion) , A0A173G4P4 (Abl fusion)	Uniprot
S374	Phosphorylation		Uniprot
S377	Phosphorylation		Uniprot
T385	Phosphorylation		Uniprot
T410	Phosphorylation		Uniprot
S429	Phosphorylation		Uniprot
T432	Phosphorylation		Uniprot
Y436	Phosphorylation		Uniprot
Y455	Phosphorylation		Uniprot
S459	Phosphorylation		Uniprot
S461	Phosphorylation		Uniprot
S462	Phosphorylation		Uniprot
S463	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
S468	Phosphorylation		Uniprot
S473	Phosphorylation		Uniprot
K486	Ubiquitination		Uniprot
S488	Phosphorylation		Uniprot
K494	Ubiquitination		Uniprot
Y513	Phosphorylation		Uniprot
S515	Phosphorylation		Uniprot
S540	Phosphorylation		Uniprot
K549	Ubiquitination		Uniprot
Y554	Phosphorylation		Uniprot
K558	Ubiquitination		Uniprot
Y561	Phosphorylation		Uniprot
S572	Phosphorylation		Uniprot
K583	Ubiquitination		Uniprot
S586	Phosphorylation		Uniprot
Y591	Phosphorylation		Uniprot
Y598	Phosphorylation		Uniprot
S626	Phosphorylation		Uniprot
T634	Phosphorylation		Uniprot
T635	Phosphorylation		Uniprot
K636	Ubiquitination		Uniprot
S638	Phosphorylation		Uniprot
T641	Phosphorylation		Uniprot
Y644	Phosphorylation		Uniprot
K645	Ubiquitination		Uniprot
T651	Phosphorylation		Uniprot
S653	Phosphorylation		Uniprot
T654	Phosphorylation		Uniprot
K662	Ubiquitination		Uniprot
T693	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot
Y751	Phosphorylation		Uniprot
Y756	Phosphorylation		Uniprot
K795	Ubiquitination		Uniprot
S843	Phosphorylation		Uniprot
Y844	Phosphorylation		Uniprot
T845	Phosphorylation		Uniprot
Y852	Phosphorylation		Uniprot
S871	Phosphorylation		Uniprot
S894	Phosphorylation		Uniprot
T898	Phosphorylation		Uniprot
S906	Phosphorylation		Uniprot
Y910	Phosphorylation		Uniprot
S919	Phosphorylation		Uniprot
T921	Phosphorylation		Uniprot
K1000	Ubiquitination		Uniprot
T1018	Phosphorylation		Uniprot
S1030	Phosphorylation		Uniprot
S1035	Phosphorylation		Uniprot
S1039	Phosphorylation		Uniprot
K1047	Ubiquitination		Uniprot
T1049	Phosphorylation		Uniprot
K1061	Ubiquitination		Uniprot
K1066	Ubiquitination		Uniprot
Y1069	Phosphorylation		Uniprot
Y1089	Phosphorylation		Uniprot
K1102	Ubiquitination		Uniprot
K1197	Ubiquitination		Uniprot
S1264	Phosphorylation		Uniprot

PTMs - P11274 As Enzyme

P11274

Substrate	Site	Source
P27348 (YWHAQ)	S232	Uniprot
P55196 (AFDN)	T909	Uniprot
P63104 (YWHAZ)	T232	Uniprot
Q15366 (PCBP2)	S173	Uniprot
Q15366 (PCBP2)	S189	Uniprot
Q15366 (PCBP2)	T213	Uniprot
Q15366 (PCBP2)	S272	Uniprot

Research Backgrounds

P11274_BCR_HUMAN

Function:

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The amino terminus contains an intrinsic kinase activity. Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity. Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation.

PTMs:

Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.

Subcellular Location:

Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Axon. Cell junction>Synapse.

Subunit Structure:

Homotetramer. Interacts with PDZK1. May interact with CCPG1 (By similarity). Interacts with FES/FPS, ABL1, PIK3R1 and GRB2. Interacts with HCK. Interacts with SH2D5. Interacts with DLG4.

Family&Domains:

The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.

The DH domain is involved in interaction with CCPG1.

The amino terminus contains an intrinsic kinase activity. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The C-terminus is a Rho-GAP domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. The protein has a unique structure having two opposing regulatory activities toward small GTP-binding proteins.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

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AFfirm™ Bcr Mouse Monoclonal Antibody - #BF8945