AFfirm™ p130 Cas Mouse Monoclonal Antibody - #BF8912
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti estrogen resistance 1 protein; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; CAS1; Cass1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; FLJ12176; FLJ45059; p130cas;
Immunogens
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
- P56945 BCAR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERVKELGHSTQQFRRVLGQLAAA
PTMs - P56945 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Y12 | Phosphorylation | P51813 (BMX) | Uniprot |
| S18 | Phosphorylation | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | P11362 (FGFR1) , P12931 (SRC) | Uniprot |
| S134 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| Y165 | Phosphorylation | Q13882 (PTK6) , Q07912 (TNK2) | Uniprot |
| Y179 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y234 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y249 | Phosphorylation | Q13882 (PTK6) , P11362 (FGFR1) , P12931 (SRC) , Q07912 (TNK2) | Uniprot |
| S260 | Phosphorylation | Uniprot | |
| Y262 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y267 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| T269 | Phosphorylation | Uniprot | |
| Y287 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| S292 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | P11362 (FGFR1) , Q13882 (PTK6) | Uniprot |
| S311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| Y327 | Phosphorylation | P11362 (FGFR1) , Q13882 (PTK6) | Uniprot |
| S355 | Phosphorylation | Uniprot | |
| Y362 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| Y372 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Q13882 (PTK6) | Uniprot |
| S407 | Phosphorylation | Uniprot | |
| Y410 | Phosphorylation | Q05397 (PTK2) , P11362 (FGFR1) , Q07912 (TNK2) | Uniprot |
| S428 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| Y653 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y664 | Phosphorylation | Q13882 (PTK6) , P12931 (SRC) | Uniprot |
| Y666 | Phosphorylation | P12931 (SRC) | Uniprot |
| K693 | Ubiquitination | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| Y815 | Phosphorylation | Uniprot | |
| K831 | Ubiquitination | Uniprot | |
| K851 | Ubiquitination | Uniprot |
Research Backgrounds
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128.
Cell junction>Focal adhesion. Cytoplasm.
Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1 and SH2D3C. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1. Part of a collagen-stimulated complex involved in cell migration made of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with TNK2 via SH3 domains. Interacts with PTK2B/PYK2.
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9 (By similarity).
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
Belongs to the CAS family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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