ATP6V0A4 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	ATP6V0A4 Mouse Monoclonal Antibody
Catalog:	BF8904
Description:	Mouse monoclonal antibody to ATP6V0A4
Application:	WB
Reactivity:	Human
Prediction:	Mouse
Mol.Wt.:	70,95kD; 96kD(Calculated).
Uniprot:	Q9HBG4

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8904]

Specificity:

ATP6V0A4 Mouse Monoclonal Antibody detects endogenous levels of ATP6V0A4.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A4; ATP6N1B; ATP6N2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) noncatalytic accessory protein 2, 38kD; ATPase, H+ transporting, lysosomal V0 subunit a4; ATPase, H+ TRANSPORTING, LYSOSOMAL, NONCATALYTIC ACCESSORY PROTEIN 1B; MGC130016; MGC130017; RDRTA2; RTA1C; RTADR; STV1; V ATPase 116 kDa isoform a4; VACUOLAR PROTEIN PUMP, SUBUNIT 2; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; VPH1; VPP2;

Immunogens

Immunogen:

A synthesized peptide derived from human ATP6V0A4.

Uniprot:

Q9HBG4(VPP4_HUMAN) >>Visit HPA database.

Gene(ID):

ATP6V0A4(50617)

Expression:

Q9HBG4 VPP4_HUMAN:

Expressed in adult and fetal kidney. Found in the inner ear.

Sequence:

Q9HBG4 VPP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTSADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE

Research Backgrounds

Q9HBG4_VPP4_HUMAN

Function:

Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney.

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein.
Note: Present at high density almost exclusively on the apical surface of alpha-intercalated cells in the cortical collecting ducts of the distal nephron.

Tissue Specificity:

Expressed in adult and fetal kidney. Found in the inner ear.

Subunit Structure:

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

Family&Domains:

Belongs to the V-ATPase 116 kDa subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Collecting duct acid secretion.

Restrictive clause

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AFfirm™ ATP6V0A4 Mouse Monoclonal Antibody - #BF8904