AFfirm™ PTRF Mouse Monoclonal Antibody - #BF8886

Product: PTRF Mouse Monoclonal Antibody
Catalog: BF8886
Description: Mouse monoclonal antibody to PTRF
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: Q6NZI2

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8886]
Specificity:
PTRF Mouse Monoclonal Antibody detects endogenous levels of total PTRF.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2310075E07Rik; AW546441; Cavin 1; Cavin; Cavin-1; CAVIN1; CGL4; FKSG13; FLJ90031; MGC118550; OTTMUSP00000002049; Polymerase I and transcript release factor; Ptrf; PTRF_HUMAN; RNA polymerase I and transcript release factor; RP23-279L23.6; TTF I interacting peptide 12;

Immunogens

Immunogen:
Uniprot:

Q6NZI2(CAVN1_HUMAN) >>Visit HPA database.

Gene(ID):
CAVIN1(284119)
Sequence:
MEDPTLYIVERPLPGYPDAEAPEPSSAGAQAAEEPSGAGSEELIKSDQVNGVLVLSLLDKIIGAVDQIQLTQAQLEERQAEMEGAVQSIQGELSKLGKAHATTSNTVSKLLEKVRKVSVNVKTVRGSLERQAGQIKKLEVNEAELLRRRNFKVMIYQDEVKLPAKLSISKSLKESEALPEKEGEELGEGERPEEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD

PTMs - Q6NZI2 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S26 Phosphorylation
S36 Phosphorylation
S40 Phosphorylation
S46 Phosphorylation
K60 Ubiquitination
S88 Phosphorylation
S94 Phosphorylation
K95 Ubiquitination
K98 Ubiquitination
T103 Phosphorylation
S104 Phosphorylation
S108 Phosphorylation
K109 Acetylation
K109 Ubiquitination
K113 Ubiquitination
K116 Sumoylation
K116 Ubiquitination
S118 Phosphorylation
K122 Ubiquitination
S127 Phosphorylation
K137 Ubiquitination
K152 Acetylation
K152 Ubiquitination
Y156 Phosphorylation
K161 Acetylation
K161 Sumoylation
K161 Ubiquitination
K165 Acetylation
K165 Ubiquitination
S167 Phosphorylation
S169 Phosphorylation
K170 Sumoylation
K170 Ubiquitination
S171 Phosphorylation
K173 Sumoylation
K173 Ubiquitination
S175 Phosphorylation
K181 Ubiquitination
S202 Phosphorylation
S203 Phosphorylation
S216 Phosphorylation
S237 Phosphorylation
K254 Ubiquitination
K258 Ubiquitination
T259 Phosphorylation
K260 Ubiquitination
K265 Ubiquitination
T269 Phosphorylation
K272 Ubiquitination
K276 Ubiquitination
K299 Ubiquitination
S300 Phosphorylation Q96GD4 (AURKB)
T302 Phosphorylation
Y308 Phosphorylation
K312 Ubiquitination
T313 Phosphorylation
Y316 Phosphorylation
K317 Ubiquitination
K326 Acetylation
K326 Ubiquitination
K327 Acetylation
K337 Ubiquitination
S365 Phosphorylation
S366 Phosphorylation
T376 Phosphorylation
S379 Phosphorylation
S387 Phosphorylation
S389 Phosphorylation

Research Backgrounds

Function:

Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues. Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).

PTMs:

Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity. Phosphorylation at Tyr-156 is essential for its functionin the regulation of ribosomal transcriptional activity.

Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.

Monoubiquitinated.

Subcellular Location:

Membrane>Caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm>Cytosol. Mitochondrion. Nucleus.
Note: Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the CAVIN complex composed of CAVIN1, CAVIN2, CAVIN3 and CAVIN4. Homotrimer (By similarity). Interacts with TTF1. Interacts with RNA polymerase I. Binds the 3' end of pre-rRNA. Interacts with transcription factor ZNF148 (By similarity). Interacts with LIPE in the adipocyte cytoplasm. Interacts with CAV1 and CAVIN3 (By similarity). Interacts with CAVIN2. Interacts with CAVIN4 and CAV3.

Family&Domains:

The leucine-zipper domain 1 is essential for its localization in the caveolae.

Belongs to the CAVIN family.

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