PTP alpha Mouse Monoclonal Antibody

Product:	PTP alpha Mouse Monoclonal Antibody
Catalog:	BF8879
Description:	Mouse monoclonal antibody to PTP alpha
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	90kDa; 91kD(Calculated).
Uniprot:	P18433

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8879]

Specificity:

PTP alpha Mouse Monoclonal Antibody detects endogenous levels of total PTP alpha.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HEPTP; HLPR; HPTP alpha; HPTPA; HPTPalpha; Leukocyte common antigen related peptide; LRP; Protein tyrosine phosphatase alpha; Protein tyrosine phosphatase receptor type A; Protein tyrosine phosphatase receptor type alpha polypeptide; Protein tyrosine phosphate; Protein-tyrosine phosphatase alpha; PTPA; PTPalpha; PTPase alpha; PTPLCA related phosphatase; Ptpra; PTPRA_HUMAN; PTPRL 2; PTPRL2; PTRA; R PTP alpha; R-PTP-alpha; Receptor type tyrosine protein phosphatase alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTPA; Tyrosine phosphatase alpha;

Immunogens

Immunogen:

Uniprot:

P18433(PTPRA_HUMAN) >>Visit HPA database.

Gene(ID):

PTPRA(5786)

Sequence:

P18433 PTPRA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDSWFILVLLGSGLICVSANNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSGNSDSKDRRDETPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYPPLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK

PTMs - P18433 As Substrate

P18433

Site	PTM Type	Enzyme	Source
S18	Phosphorylation		Uniprot
S187	Phosphorylation		Uniprot
S189	Phosphorylation	Q05655 (PRKCD)	Uniprot
S204	Phosphorylation	Q9UQM7 (CAMK2A) , Q05655 (PRKCD)	Uniprot
S211	Phosphorylation		Uniprot
S213	Phosphorylation	Q05655 (PRKCD)	Uniprot
T214	Phosphorylation		Uniprot
Y218	Phosphorylation		Uniprot
K225	Ubiquitination		Uniprot
Y271	Phosphorylation		Uniprot
S293	Phosphorylation		Uniprot
K307	Ubiquitination		Uniprot
S407	Phosphorylation		Uniprot
Y522	Phosphorylation		Uniprot
K524	Ubiquitination		Uniprot
S542	Phosphorylation		Uniprot
T552	Phosphorylation		Uniprot
K560	Ubiquitination		Uniprot
Y588	Phosphorylation		Uniprot
K765	Acetylation		Uniprot
S766	Phosphorylation		Uniprot
Y791	Phosphorylation		Uniprot
S796	Phosphorylation		Uniprot
Y798	Phosphorylation	P12931 (SRC)	Uniprot

Research Backgrounds

P18433_PTPRA_HUMAN

Function:

Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (By similarity). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (By similarity).

PTMs:

Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion.
Note: Localizes to focal adhesion sites following integrin engagement.

Subunit Structure:

Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain), and SRC. Within the complex, interacts (when phosphorylated on Tyr-798) with BCAR3 (via SH2 domain). Interacts with GRB2.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ PTP alpha Mouse Monoclonal Antibody - #BF8879