AFfirm™ EIF4A1 Mouse Monoclonal Antibody - #BF8876
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN;
Immunogens
- P60842 IF4A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
PTMs - P60842 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Methylation | Uniprot | |
| K54 | Sumoylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| C66 | S-Nitrosylation | Uniprot | |
| K68 | Sumoylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K146 | Sumoylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| R168 | Methylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Methylation | Uniprot | |
| K193 | Sumoylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| K202 | Sumoylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| K225 | Sumoylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K238 | Sumoylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| R255 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K291 | Sumoylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K309 | Acetylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| R316 | Methylation | Uniprot | |
| R353 | Methylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K381 | Acetylation | Uniprot | |
| K381 | Ubiquitination | Uniprot |
Research Backgrounds
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL69.
Belongs to the DEAD box helicase family. eIF4A subfamily.
Research Fields
· Genetic Information Processing > Translation > RNA transport.
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