AFfirm™ BCL11A Mouse Monoclonal Antibody - #BF8875

Product: BCL11A Mouse Monoclonal Antibody
Catalog: BF8875
Description: Mouse monoclonal antibody to BCL11A
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90~150kDa; 91kD(Calculated).
Uniprot: Q9H165

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8875]
Specificity:
BCL11A Mouse Monoclonal Antibody detects endogenous levels of total BCL11A.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810047E18Rik; B cell CLL/lymphoma 11A (zinc finger protein); B cell CLL/lymphoma 11A (zinc finger protein) isoform 2; B-cell CLL/lymphoma 11A; B-cell lymphoma/leukemia 11A; BC11A_HUMAN; BCL-11A; BCL11A; BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1; BCL11A L; BCL11A S; BCL11A XL; BCL11a-M; BCL11AL; BCL11AS; BCL11AXL; C2H2 type zinc finger protein; COUP TF interacting protein 1; COUP-TF-interacting protein 1; CTIP1; CTIP1, mouse, homolog of; D930021L15Rik; Ecotropic viral integration site 9; Ecotropic viral integration site 9 homolog; Ecotropic viral integration site 9 protein; Ecotropic viral integration site 9 protein homolog; EVI-9; Evi9; Evi9, mouse, homolog of; FLJ10173; FLJ34997; HBFQTL5; KIAA1809; mKIAA1809; OTTHUMP00000159788; OTTHUMP00000159789; OTTHUMP00000201250; OTTHUMP00000202084; Zinc finger protein 856; ZNF856;

Immunogens

Immunogen:
Uniprot:

Q9H165(BC11A_HUMAN) >>Visit HPA database.

Gene(ID):
BCL11A(53335)
Expression:
Q9H165 BC11A_HUMAN:

Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.

Sequence:
MSRRKQGKPQHLSKREFSPEPLEAILTDDEPDHGPLGAPEGDHDLLTCGQCQMNFPLGDILIFIEHKRKQCNGSLCLEKAVDKPPSPSPIEMKKASNPVEVGIQVTPEDDDCLSTSSRGICPKQEHIADKLLHWRGLSSPRSAHGALIPTPGMSAEYAPQGICKDEPSSYTCTTCKQPFTSAWFLLQHAQNTHGLRIYLESEHGSPLTPRVGIPSGLGAECPSQPPLHGIHIADNNPFNLLRIPGSVSREASGLAEGRFPPTPPLFSPPPRHHLDPHRIERLGAEEMALATHHPSAFDRVLRLNPMAMEPPAMDFSRRLRELAGNTSSPPLSPGRPSPMQRLLQPFQPGSKPPFLATPPLPPLQSAPPPSQPPVKSKSCEFCGKTFKFQSNLVVHRRSHTGEKPYKCNLCDHACTQASKLKRHMKTHMHKSSPMTVKSDDGLSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDDEEEEEEEEEEEEELTESERVDYGFGLSLEAARHHENSSRGAVVGVGDESRALPDVMQGMVLSSMQHFSEAFHQVLGEKHKRGHLAEAEGHRDTCDEDSVAGESDRIDDGTVNGRGCSPGESASGGLSKKLLLGSPSSLSPFSKRIKLEKEFDLPPAAMPNTENVYSQWLAGYAASRQLKDPFLSFGDSRQSPFASSSEHSSENGSLRFSTPPGELDGGISGRSGTGSGGSTPHISGPGPGRPSSKEGRRSDTCEYCGKVFKNCSNLTVHRRSHTGERPYKCELCNYACAQSSKLTRHMKTHGQVGKDVYKCEICKMPFSVYSTLEKHMKKWHSDRVLNNDIKTE

PTMs - Q9H165 As Substrate

Site PTM Type Enzyme
S86 Phosphorylation
S88 Phosphorylation
K123 Ubiquitination
K130 Ubiquitination
T150 Phosphorylation
Y198 Phosphorylation
S201 Phosphorylation
S205 Phosphorylation
T208 Phosphorylation
T262 Phosphorylation
S267 Phosphorylation
S328 Phosphorylation
S332 Phosphorylation
T357 Phosphorylation
K377 Ubiquitination
K384 Acetylation
K384 Ubiquitination
T400 Phosphorylation
T426 Phosphorylation
S432 Phosphorylation
T435 Phosphorylation
S446 Phosphorylation
S447 Phosphorylation
T584 Phosphorylation
S589 Phosphorylation
S594 Phosphorylation
S608 Phosphorylation
S612 Phosphorylation
S618 Phosphorylation
K619 Ubiquitination
K620 Ubiquitination
S625 Phosphorylation
S627 Phosphorylation
S628 Phosphorylation
S630 Phosphorylation
S633 Phosphorylation
K634 Ubiquitination
K670 Ubiquitination
T701 Phosphorylation
S714 Phosphorylation
S718 Phosphorylation
S721 Phosphorylation
T722 Phosphorylation
K736 Ubiquitination
S741 Phosphorylation
T743 Phosphorylation
K749 Ubiquitination
K752 Ubiquitination
S755 Phosphorylation
T765 Phosphorylation
K771 Ubiquitination
K784 Ubiquitination

Research Backgrounds

Function:

Transcription factor associated with the BAF SWI/SNF chromatin remodeling complex (By similarity). Repressor of fetal hemoglobin (HbF) level. Involved in brain development. May play a role in hematopoiesis. Essential factor in lymphopoiesis required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity).

PTMs:

Sumoylated with SUMO1.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles (By similarity).

Cytoplasm. Nucleus.
Note: Predominantly localized in the nucleus in nuclear paraspeckles.

Cytoplasm. Nucleus.
Note: Predominantly localized in the cytoplasm in the absence of interaction with isoform 1 and isoform 2. In presence of isoform 1 or isoform 2, translocates from the cytoplasm into nuclear paraspeckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.

Subunit Structure:

Interacts with TFCOUP1, PIAS3, ARP1 and EAR2 (By similarity). Isoform 1, isoform 2 and isoform 3 form homodimers and heterodimers. Isoform 2 interacts with TBR1.

Family&Domains:

The N-terminus is involved in protein dimerization and in transactivation of transcription.

The C-terminus of isoform 2 is necessary for isoform 2 interaction with TBR1.

Restrictive clause

 

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