HNF1A Mouse Monoclonal Antibody | Affinity Biosciences

Product:	HNF1A Mouse Monoclonal Antibody
Catalog:	BF8874
Description:	Mouse monoclonal antibody to HNF1A
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	56kDa, 70 kDa; 67kD(Calculated).
Uniprot:	P20823

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8874]

Specificity:

HNF1A Mouse Monoclonal Antibody detects endogenous levels of total HNF1A.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic;

Immunogens

Immunogen:

Uniprot:

P20823(HNF1A_HUMAN) >>Visit HPA database.

Gene(ID):

HNF1A(6927)

Expression:

P20823 HNF1A_HUMAN:

Liver.

Sequence:

P20823 HNF1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLDKGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ

PTMs - P20823 As Substrate

P20823

Site	PTM Type	Enzyme	Source
T67	Phosphorylation		Uniprot
S70	Phosphorylation		Uniprot
T74	Phosphorylation		Uniprot
T82	Phosphorylation		Uniprot
S93	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
S249	Phosphorylation	Q9Y463 (DYRK1B) , Q13315 (ATM)	Uniprot

Research Backgrounds

P20823_HNF1A_HUMAN

Function:

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Liver.

Subunit Structure:

Binds DNA as a dimer. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity). Interacts with PCBD1. Interacts with BHLHE41 (By similarity).

Family&Domains:

Belongs to the HNF1 homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

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For Research Use Only.
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AFfirm™ HNF1A Mouse Monoclonal Antibody - #BF8874