AFfirm™ UBF1 Mouse Monoclonal Antibody - #BF8873
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Product Info
Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Monoclonal [AFfirm8873]
Specificity:
UBF1 Mouse Monoclonal Antibody detects endogenous levels of total UBF1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P17480 UBF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN
PTMs - P17480 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| T117 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K126 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K160 | Sumoylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| T201 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| Y207 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K232 | Methylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K254 | Methylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K284 | Acetylation | Uniprot | |
| K284 | Sumoylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K385 | Acetylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S392 | Phosphorylation | Uniprot | |
| K394 | Acetylation | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| R429 | Methylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| R443 | Methylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| Y456 | Phosphorylation | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | P24941 (CDK2) , P11802 (CDK4) | Uniprot |
| S495 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| K559 | Acetylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| R642 | Methylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| Y648 | Phosphorylation | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
PTMs:
Phosphorylated and activated by PIK3CA.
Subcellular Location:
Nucleus>Nucleolus.
Subunit Structure:
Homodimer (By similarity). Interacts with TBP. Interacts with TAF1A. Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33. Interacts with PHF6. Interacts with CEBPA (isoform 1 and isoform 4). Interacts with DDX11. Interacts with NOP53.
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For Research Use Only.
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