AFfirm™ EPHA1 Mouse Monoclonal Antibody - #BF8866

Product: EPHA1 Mouse Monoclonal Antibody
Catalog: BF8866
Description: Mouse monoclonal antibody to EPHA1
Application: WB
Reactivity: Human
Prediction: Mouse, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 108 KD; 108kD(Calculated).
Uniprot: P21709

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8866]
Specificity:
EPHA1 Mouse Monoclonal Antibody detects endogenous levels of total EPHA1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EPH; EPH receptor A1; EPH tyrosine kinase 1; EPH tyrosine kinase; Epha1; EPHA1_HUMAN; Ephrin receptor Eph A1; Ephrin type A receptor 1; Ephrin type-A receptor 1; EPHT 1; EPHT; EPHT1; Erythropoietin producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; Esk; hEpha1; MGC163163; Oncogene EPH; Soluble EPHA1 variant 1; Soluble EPHA1 variant 2; Tyrosine protein kinase receptor EPH; Tyrosine-protein kinase receptor EPH;

Immunogens

Immunogen:
Uniprot:

P21709(EPHA1_HUMAN) >>Visit HPA database.

Gene(ID):
EPHA1(2041)
Expression:
P21709 EPHA1_HUMAN:

Overexpressed in several carcinomas.

Sequence:
MERRWPLGLGLVLLLCAPLPPGARAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCPMQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLVSGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNGLAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVACPSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSLRWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVEAQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDEERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAVIFGLLLGAALLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVIGEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRMTLRLPSLSGSDGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD

PTMs - P21709 As Substrate

Site PTM Type Enzyme
T33 Phosphorylation
S34 Phosphorylation
S153 Phosphorylation
T155 Phosphorylation
K593 Ubiquitination
K597 Ubiquitination
Y599 Phosphorylation
Y605 Phosphorylation
T780 Phosphorylation
Y781 Phosphorylation
T783 Phosphorylation
K787 Ubiquitination
T805 Phosphorylation
S811 Phosphorylation
T892 Phosphorylation
S906 Phosphorylation
S908 Phosphorylation
S910 Phosphorylation
S919 Phosphorylation

Research Backgrounds

Function:

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

PTMs:

Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.

Ubiquitinated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Overexpressed in several carcinomas.

Subunit Structure:

Homodimer. Forms a signaling complex with LCK; PTK2B/PYK2 and PI3-kinase upon activation by EFNA1; regulates T-lymphocytes migration. Interacts (via SAM domain) with ILK (via ANK repeats); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts (kinase activity-dependent) with PTK2/FAK1.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

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