MAT1A Mouse Monoclonal Antibody | Affinity Biosciences

Product:	MAT1A Mouse Monoclonal Antibody
Catalog:	BF8854
Description:	Mouse monoclonal antibody to MAT1A
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	44-55 kDa; 44kD(Calculated).
Uniprot:	Q00266

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8854]

Specificity:

MAT1A Mouse Monoclonal Antibody detects endogenous levels of total MAT1A.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AdoMet; AdoMet synthetase 1; AI046368; Ams; MAT; MAT I/III; MATA1; Methionine adenosyltransferase 1; Methionine adenosyltransferase I, alpha; Methionine adenosyltransferase I/III; MGC108563; S adenosylmethionine synthetase isoform type 1; SADE; SAMS; SAMS1;

Immunogens

Immunogen:

Uniprot:

Q00266(METK1_HUMAN) >>Visit HPA database.

Gene(ID):

MAT1A(4143)

Expression:

Q00266 METK1_HUMAN:

Expressed in liver.

Sequence:

Q00266 METK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF

PTMs - Q00266 As Substrate

Q00266

Site	PTM Type	Source
S73	Phosphorylation	Uniprot
K223	Ubiquitination	Uniprot
R264	Methylation	Uniprot
K285	Ubiquitination	Uniprot
K289	Ubiquitination	Uniprot
S293	Phosphorylation	Uniprot
K303	Ubiquitination	Uniprot
T341	Phosphorylation	Uniprot
K351	Ubiquitination	Uniprot

Research Backgrounds

Q00266_METK1_HUMAN

Function:

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

PTMs:

S-nitrosylation of Cys-120 inactivates the enzyme.

An intrachain disulfide bond can be formed. The protein structure shows that the relevant Cys residues are in a position that would permit formation of a disulfide bond.

Tissue Specificity:

Expressed in liver.

Subunit Structure:

Homotetramer (MAT-I); dimer of dimers. Homodimer (MAT-III) (By similarity).

Family&Domains:

Belongs to the AdoMet synthase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ MAT1A Mouse Monoclonal Antibody - #BF8854