AFfirm™ NASP Mouse Monoclonal Antibody - #BF8851
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Protocols
Product Info
Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Monoclonal [AFfirm8851]
Specificity:
NASP Mouse Monoclonal Antibody detects endogenous levels of total NASP.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
5033430J04Rik; AI131596; AI317140; D4Ertd767e; DKFZp547F162; Epcs32; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; MGC93869; NASP; NASP_HUMAN; nuclear autoantigenic sperm protein (histone binding); Nuclear autoantigenic sperm protein; PRO1999; RP23 233B9.9;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- P49321 NASP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDEEAREELREQVYDAMGEKEEAKKTEDKSLAKPETDKEQDSEMEKGGREDMDISKSAEEPQEKVDLTLDWLTETSEEAKGGAAPEGPNEAEVTSGKPEQEVPDAEEEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEEQPVVTLEKQGTAVEVEAESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAEESPEVTTEAAEASAVEAGSEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIERLTETKDGSGLEEKVRAKLVPSQEETKLSVEESEAAGDGVDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC
PTMs - P49321 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K20 | Acetylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| R140 | Methylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| C254 | S-Nitrosylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| K450 | Acetylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| K455 | Acetylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| T490 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| Y540 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| Y575 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| R617 | Methylation | Uniprot | |
| K626 | Ubiquitination | Uniprot | |
| K636 | Sumoylation | Uniprot | |
| K643 | Acetylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| T683 | Phosphorylation | Uniprot | |
| S689 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| K698 | Acetylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| K721 | Methylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| K733 | Acetylation | Uniprot | |
| K734 | Acetylation | Uniprot | |
| K736 | Acetylation | Uniprot | |
| K736 | Sumoylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S756 | Phosphorylation | Uniprot | |
| C788 | S-Nitrosylation | Uniprot |
Research Backgrounds
Function:
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Isoform 1 is testis- and sperm-specific.
Subunit Structure:
Binds to linker H1 histones but not to core histones (By similarity). Interacts with histones H2A, H2B, H3 and H4. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).
Family&Domains:
Belongs to the NASP family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.