MDH1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	MDH1 Mouse Monoclonal Antibody
Catalog:	BF8844
Description:	Mouse monoclonal antibody to MDH1
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	36 kDa; 36kD(Calculated).
Uniprot:	P40925

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8844]

Specificity:

MDH1 Mouse Monoclonal Antibody detects endogenous levels of total MDH1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase; Malate dehydrogenase cytoplasmic; MDH s; mdh1; MDHA; MDHC_HUMAN; MDHs; MGC:1375; MOR2; Soluble malate dehydrogenase;

Immunogens

Immunogen:

Uniprot:

P40925(MDHC_HUMAN) >>Visit HPA database.

Gene(ID):

MDH1(4190)

Sequence:

P40925 MDHC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

PTMs - P40925 As Substrate

P40925

Site	PTM Type	Source
S2	Acetylation	Uniprot
Y22	Phosphorylation	Uniprot
T71	Phosphorylation	Uniprot
K73	Acetylation	Uniprot
K73	Ubiquitination	Uniprot
S89	Phosphorylation	Uniprot
K103	Acetylation	Uniprot
K103	Ubiquitination	Uniprot
K107	Acetylation	Uniprot
K107	Ubiquitination	Uniprot
K110	Methylation	Uniprot
K110	Ubiquitination	Uniprot
S111	Phosphorylation	Uniprot
K118	Acetylation	Uniprot
K118	Ubiquitination	Uniprot
Y119	Phosphorylation	Uniprot
K121	Acetylation	Uniprot
K125	Ubiquitination	Uniprot
C137	S-Nitrosylation	Uniprot
K142	Acetylation	Uniprot
K142	Ubiquitination	Uniprot
K149	Ubiquitination	Uniprot
C154	S-Nitrosylation	Uniprot
K164	Ubiquitination	Uniprot
K170	Ubiquitination	Uniprot
T174	Phosphorylation	Uniprot
K179	Ubiquitination	Uniprot
S188	Phosphorylation	Uniprot
S189	Phosphorylation	Uniprot
T190	Phosphorylation	Uniprot
Y192	Phosphorylation	Uniprot
K199	Ubiquitination	Uniprot
K205	Ubiquitination	Uniprot
Y210	Phosphorylation	Uniprot
K214	Acetylation	Uniprot
K214	Ubiquitination	Uniprot
S217	Phosphorylation	Uniprot
K220	Ubiquitination	Uniprot
R230	Methylation	Uniprot
K236	Ubiquitination	Uniprot
K239	Ubiquitination	Uniprot
S241	Phosphorylation	Uniprot
S242	Phosphorylation	Uniprot
S245	Phosphorylation	Uniprot
T296	Phosphorylation	Uniprot
K298	Acetylation	Uniprot
K298	Ubiquitination	Uniprot
S309	Phosphorylation	Uniprot
K312	Ubiquitination	Uniprot
K318	Ubiquitination	Uniprot
T321	Phosphorylation	Uniprot
K324	Ubiquitination	Uniprot
S326	Phosphorylation	Uniprot
S332	Phosphorylation	Uniprot
S333	Phosphorylation	Uniprot

Research Backgrounds

P40925_MDHC_HUMAN

PTMs:

ISGylated.

Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Research Fields

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ MDH1 Mouse Monoclonal Antibody - #BF8844