Flotillin 1 Mouse Monoclonal Antibody

Product:	Flotillin 1 Mouse Monoclonal Antibody
Catalog:	BF8826
Description:	Mouse monoclonal antibody to Flotillin 1
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	47 kDa; 47kD(Calculated).
Uniprot:	O75955

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8826]

Specificity:

Flotillin 1 Mouse Monoclonal Antibody detects endogenous levels of total Flotillin 1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin-1; Flotillin1; Integral membrane component of caveolae; Reggie 2;

Immunogens

Immunogen:

Uniprot:

O75955(FLOT1_HUMAN) >>Visit HPA database.

Gene(ID):

FLOT1(10211)

Sequence:

O75955 FLOT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA

PTMs - O75955 As Substrate

O75955

Site	PTM Type	Enzyme	Source
S19	Phosphorylation		Uniprot
K51	Ubiquitination		Uniprot
K54	Ubiquitination		Uniprot
Y56	Phosphorylation	P12931 (SRC)	Uniprot
K78	Ubiquitination		Uniprot
K122	Ubiquitination		Uniprot
K126	Ubiquitination		Uniprot
S128	Phosphorylation		Uniprot
Y149	Phosphorylation	P12931 (SRC)	Uniprot
K152	Ubiquitination		Uniprot
Y160	Phosphorylation	P06241 (FYN)	Uniprot
S163	Phosphorylation		Uniprot
K166	Ubiquitination		Uniprot
K174	Ubiquitination		Uniprot
K184	Ubiquitination		Uniprot
K195	Ubiquitination		Uniprot
K198	Ubiquitination		Uniprot
Y203	Phosphorylation		Uniprot
S205	Phosphorylation		Uniprot
K211	Ubiquitination		Uniprot
Y216	Phosphorylation		Uniprot
K220	Methylation		Uniprot
K220	Ubiquitination		Uniprot
Y223	Phosphorylation		Uniprot
Y238	Phosphorylation		Uniprot
K244	Ubiquitination		Uniprot
Y292	Phosphorylation		Uniprot
K293	Ubiquitination		Uniprot
K302	Ubiquitination		Uniprot
S315	Phosphorylation		Uniprot
K341	Ubiquitination		Uniprot
Y348	Phosphorylation		Uniprot
S383	Phosphorylation		Uniprot
S385	Phosphorylation		Uniprot
T387	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
S407	Phosphorylation		Uniprot
K422	Ubiquitination		Uniprot

Research Backgrounds

O75955_FLOT1_HUMAN

Function:

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Endosome. Membrane>Caveola>Peripheral membrane protein. Melanosome. Membrane raft.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity).

Subunit Structure:

Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS.

Family&Domains:

Belongs to the band 7/mec-2 family. Flotillin subfamily.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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AFfirm™ Flotillin 1 Mouse Monoclonal Antibody - #BF8826