AFfirm™ ALX1 Mouse Monoclonal Antibody - #BF8779

Product: ALX1 Mouse Monoclonal Antibody
Catalog: BF8779
Description: Mouse monoclonal antibody to ALX1
Application: WB
Reactivity: Human
Prediction: Mouse, Rat
Mol.Wt.: 37kD(Calculated).
Uniprot: Q15699

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8779]
Specificity:
ALX1 Mouse Monoclonal Antibody detects endogenous levels of ALX1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALX homeobox 1; ALX homeobox protein 1; ALX1; ALX1_HUMAN; CART 1; CART-1; CART1; Cartilage homeoprotein 1; Cartilage paired class homeoprotein 1; FND3;

Immunogens

Immunogen:

A synthesized peptide derived from human ALX1.

Uniprot:

Q15699(ALX1_HUMAN) >>Visit HPA database.

Gene(ID):
ALX1(8092)
Expression:
Q15699 ALX1_HUMAN:

Cartilage and cervix tissue.

Sequence:
MEFLSEKFALKSPPSKNSDFYMGAGGPLEHVMETLDNESFYSKASAGKCVQAFGPLPRAEHHVRLERTSPCQDSSVNYGITKVEGQPLHTELNRAMDNCNSLRMSPVKGMQEKGELDELGDKCDSNVSSSKKRRHRTTFTSLQLEELEKVFQKTHYPDVYVREQLALRTELTEARVQVWFQNRRAKWRKRERYGQIQQAKSHFAATYDISVLPRTDSYPQIQNNLWAGNASGGSVVTSCMLPRDTSSCMTPYSHSPRTDSSYTGFSNHQNQFSHVPLNNFFTDSLLTGATNGHAFETKPEFERRSSSIAVLRMKAKEHTANISWAM

Research Backgrounds

Function:

Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes. Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures. Early on in development, it plays a role in forebrain mesenchyme survival. May also induce epithelial to mesenchymal transition (EMT) through the expression of SNAI1.

PTMs:

Acetylated at Lys-131 by EP300; increases interaction with EP300 and stimulates ALX1 transcriptional activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Cartilage and cervix tissue.

Subunit Structure:

Binds DNA as a homodimer; required for transcriptional activation. Interacts (via homeobox domain) with EP300; acetylates ALX1 and stimulates its transcriptional activity.

Family&Domains:

The OAR motif may negatively regulate DNA-binding and therefore transcriptional activity. It is found in the C-terminal transactivation domain that stimulates transcription.

Belongs to the paired homeobox family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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