RUFY3 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	RUFY3 Mouse Monoclonal Antibody
Catalog:	BF8772
Description:	Mouse monoclonal antibody to RUFY3
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat
Mol.Wt.:	53kD(Calculated).
Uniprot:	Q7L099

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8772]

Specificity:

RUFY3 Mouse Monoclonal Antibody detects endogenous levels of RUFY3.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Protein RUFY3; rap2 interacting protein x; Rap2 interacting protein x isoform 2; Rap2-interacting protein x; RIPx; Rpipx; Rufy3; RUFY3_HUMAN; RUN and FYVE domain containing 3; Singar; Singar1; Single axon regulated protein; single axon related 1; Single axon-regulated protein;

Immunogens

Immunogen:

A synthesized peptide derived from human RUFY3.

Uniprot:

Q7L099(RUFY3_HUMAN) >>Visit HPA database.

Gene(ID):

RUFY3(22902)

Expression:

Q7L099 RUFY3_HUMAN:

Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).

Sequence:

Q7L099 RUFY3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSALTPPTDMPTPTTDKITQAAMETIYLCKFRVSMDGEWLCLRELDDISLTPDPEPTHEDPNYLMANERMNLMNMAKLSIKGLIESALNLGRTLDSDYAPLQQFFVVMEHCLKHGLKAKKTFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMKGEDLDSQVGVIDFSMYLKDGNSSKGTEGDGQITAILDQKNYVEELNRHLNATVNNLQAKVDALEKSNTKLTEELAVANNRIITLQEEMERVKEESSYILESNRKGPKQDRTAEGQALSEARKHLKEETQLRLDVEKELEMQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH

Research Backgrounds

Q7L099_RUFY3_HUMAN

Function:

Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery. Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner.

PTMs:

Phosphorylated by PAK1. Isoform 1 is partially phosphorylated (By similarity).

Subcellular Location:

Cytoplasm. Endomembrane system. Cell projection>Invadopodium. Perikaryon. Cell projection. Cell projection>Growth cone. Cell projection>Filopodium. Cell projection>Lamellipodium.
Note: Colocalizes with PAK1, F-actin, myosins and integrins in invadopodia at the cell periphery (PubMed:25766321). Colocalized with Ras-related Rab-5 proteins in cytoplasmic vesicles (PubMed:20376209). Accumulates in axon growth cones in a F-actin-dependent manner (By similarity). Colocalized with FSCN1 and F-actin at filipodia and lamellipodia of axonal growth cones (By similarity). Colocalized with DBN1 and F-actin at transitional domain of the axonal growth cone (By similarity).

Tissue Specificity:

Overexpressed in gastric cancer cells and tissues (at protein level).

Subunit Structure:

Interacts with PAK1. Interacts (via C-terminus) with Ras-related Rab-5 proteins. Interacts (via C-terminus) with Ras-related Rap-2 proteins. Interacts with PIK3CA and PIK3R1 (By similarity). Interacts (via N-terminus) with FSCN1; this interaction induces neuron axon development (By similarity). Interacts with DBN1 (By similarity).

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