AFfirm™ ANXA13 Mouse Monoclonal Antibody - #BF8733

Product: ANXA13 Mouse Monoclonal Antibody
Catalog: BF8733
Description: Mouse monoclonal antibody to ANXA13
Application: WB
Reactivity: Human
Prediction: Mouse, Rat
Mol.Wt.: 38kD; 35kD(Calculated).
Uniprot: P27216

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8733]
Specificity:
ANXA13 Mouse Monoclonal Antibody detects endogenous levels of ANXA13.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Annexin A13; Annexin XIII; Annexin, intestine-specific; Annexin-13; ANX 13; ANX A13; ANX13; ANX13_HUMAN; ANXA13; Intestine Specific Annexin; Intestine-specific annexin; ISA; MGC150460;

Immunogens

Immunogen:

A synthesized peptide derived from human ANXA13.

Uniprot:

P27216(ANX13_HUMAN) >>Visit HPA database.

Gene(ID):
ANXA13(312)
Expression:
P27216 ANX13_HUMAN:

Detected in epithelial cells in colon and jejunum (at protein level). Detected in epithelial cells in jejunum.

Sequence:
MGNRHAKASSPQGFDVDRDAKKLNKACKGMGTNEAAIIEILSGRTSDERQQIKQKYKATYGKELEEVLKSELSGNFEKTALALLDRPSEYAARQLQKAMKGLGTDESVLIEVLCTRTNKEIIAIKEAYQRLFDRSLESDVKGDTSGNLKKILVSLLQANRNEGDDVDKDLAGQDAKDLYDAGEGRWGTDELAFNEVLAKRSYKQLRATFQAYQILIGKDIEEAIEEETSGDLQKAYLTLVRCAQDCEDYFAERLYKSMKGAGTDEETLIRIVVTRAEVDLQGIKAKFQEKYQKSLSDMVRSDTSGDFRKLLVALLH

Research Backgrounds

Function:

Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner. Half-maximal membrane binding requires about 60 uM calcium. Does not bind to membranes that lack phospholipids with an acidic headgroup.

Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner, but requires higher calcium levels for membrane binding than isoform A. Half-maximal membrane binding requires about 320 uM calcium.

Subcellular Location:

Apical cell membrane. Cell membrane>Lipid-anchor. Cytoplasmic vesicle.
Note: Myristoylation anchors the protein to the membrane, but the protein displays also calcium-dependent, reversible binding to lipid membranes (PubMed:30610115). Associated with the plasma membrane of undifferentiated, proliferating crypt epithelial cells as well as differentiated villus enterocytes (PubMed:1530946).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in epithelial cells in colon and jejunum (at protein level). Detected in epithelial cells in jejunum.

Subunit Structure:

Monomer and homodimer.

Family&Domains:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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