USP21 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	USP21 Mouse Monoclonal Antibody
Catalog:	BF8692
Description:	Mouse monoclonal antibody to USP21
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat
Mol.Wt.:	65kD; 63kD(Calculated).
Uniprot:	Q9UK80

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8692]

Specificity:

USP21 Mouse Monoclonal Antibody detects endogenous levels of USP21.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deubiquitinating enzyme 21; deubiquitinating enzyme 23; EC 3.1.2.15; ESTM28; MGC3394; NEDD8-specific protease; Ubiquitin carboxyl terminal hydrolase 21; ubiquitin carboxyl terminal hydrolase 23; Ubiquitin carboxyl-terminal hydrolase 21; ubiquitin specific peptidase 21; Ubiquitin specific processing protease 21; ubiquitin specific protease 16; ubiquitin specific protease 21; ubiquitin specific protease 23; Ubiquitin thioesterase 21; Ubiquitin thiolesterase 21; ubiquitin thiolesterase 23; ubiquitin-specific protease 23, formerly; Ubiquitin-specific-processing protease 21; UBP21_HUMAN; USP16; Usp21; USP23; USP23, formerly; W53272;

Immunogens

Immunogen:

A synthesized peptide derived from human USP21.

Uniprot:

Q9UK80(UBP21_HUMAN) >>Visit HPA database.

Gene(ID):

USP21(27005)

Expression:

Q9UK80 UBP21_HUMAN:

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Sequence:

Q9UK80 UBP21_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPASGPNPMLRPLPPRPGLPDERLKKLELGRGRTSGPRPRGPLRADHGVPLPGSPPPTVALPLPSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALSRLALRPEPPTLRRSTSLRRLGGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDDKMAHHTLLLGSGHVGLRNLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL

Research Backgrounds

Q9UK80_UBP21_HUMAN

Function:

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Deubiquitinates BAZ2A/TIP5 leading to its stabilization.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Subunit Structure:

Interacts with BEND3 and BAZ2A/TIP5.

Family&Domains:

Belongs to the peptidase C19 family. USP21 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ USP21 Mouse Monoclonal Antibody - #BF8692