AFfirm™ TBC1D21 Mouse Monoclonal Antibody - #BF8680

Product: TBC1D21 Mouse Monoclonal Antibody
Catalog: BF8680
Description: Mouse monoclonal antibody to TBC1D21
Application: WB
Reactivity: Human
Prediction: Human
Mol.Wt.: 50kD; 39kD(Calculated).
Uniprot: Q8IYX1

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8680]
Specificity:
TBC1D21 Mouse Monoclonal Antibody detects endogenous levels of TBC1D21.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

male germ cell-specific expressed, containing a RabGAP domain; MGC34741; MgcRabGAP; OTTHUMP00000174928; TBC1 domain family member 21; TBC1D21; TBC21_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human TBC1D21.

Uniprot:

Q8IYX1(TBC21_HUMAN) >>Visit HPA database.

Gene(ID):
TBC1D21(161514)
Expression:
Q8IYX1 TBC21_HUMAN:

Expressed in round and elongated spermatids (at protein level). Expressed specifically in adult testis and very weakly in fetal brain.

Sequence:
MTTLSPENSLSARQSASFILVKRKPPIDKTEWDSFFDESGHLAKSRDFICVNILERGLHPFVRTEAWKFLTGYFSWQSSQDERLTVDSMRRKNYKALCQMYEKIQPLLENLHRNFTETRNNIARDIQKIYDKDPLGNVLIDKKRLEKILLLSYVCNTQAEYQQGFHEMMMLFQLMVEHDHETFWLFQFFLQKTEHSCVINIGVAKNLDMLSTLITFLDPVFAEHLKGKGAGAVQSLFPWFCFCFQRAFKSFDDVWRLWEVLLTGKPCRNFQVLVAYSMLQMVREQVLQESMGGDDILLACNNLIDLDADELISAACVVYAELIQKDVPQTLKDFFL

Research Backgrounds

Function:

May act as a GTPase-activating protein for Rab family protein(s). May be involved in acrosome formation and cytoskeletal reorganization during spermiogenesis, possibly by regulating RAB3A activity.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Acrosome. Cytoplasm>Cytoskeleton.
Note: Located at the edge of the acrosomal region, neck and annulus during spermiogenesis. Colocalizes with RAB3A at the acrosome-acroplaxome and neck regions of spermatids. Colocalizes with ACTB at the neck region in elongated spermatids.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in round and elongated spermatids (at protein level). Expressed specifically in adult testis and very weakly in fetal brain.

Subunit Structure:

Interacts with ACTB.

Family&Domains:

The arginine and glutamine fingers are critical for the GTPase-activating mechanism, they pull out Rab's 'switch 2' glutamine and insert in Rab's active site.

Restrictive clause

 

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