AFfirm™ NAB2 Mouse Monoclonal Antibody - #BF8655
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
EGR 1 binding protein 2; EGR-1-binding protein 2; EGR1 binding protein 2; MADER; Melanoma associated delayed early response protein; Melanoma-associated delayed early response protein; MGC75085; Nab 2; nab2; NAB2_HUMAN; NGFI A binding protein 2 (EGR1 binding protein 2); NGFI A binding protein 2; NGFI-A-binding protein 2; Protein MADER;
Immunogens
A synthesized peptide derived from human NAB2, corresponding to a region within C-terminal amino acids.
- Q15742 NAB2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHRAPSPTAEQPPGGGDSARRTLQPRLKPSARAMALPRTLGELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLFSQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSPPAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVESVERIFRSFPRGDAGEVTSLLKLNKKLARSVGHIFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVARESTYLSSLKGSRLHPEELGGPPLKKLKQEVGEQSHPEIQQPPPGPESYVPPYRPSLEEDSASLSGESLDGHLQAVGSCPRLTPPPADLPLALPAHGLWSRHILQQTLMDEGLRLARLVSHDRVGRLSPCVPAKPPLAEFEEGLLDRCPAPGPHPALVEGRRSSVKVEAEASRQ
PTMs - Q15742 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K169 | Sumoylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| K361 | Sumoylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K379 | Sumoylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| K517 | Sumoylation | Uniprot |
Research Backgrounds
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).
Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.
Nucleus.
Note: Isoform 2 is not localized to the nucleus.
Widely expressed at low levels. Highly expressed in melanoma cell lines.
Homomultimers may associate with EGR1 bound to DNA.
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Belongs to the NAB family.
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