AFfirm™ NAB2 Mouse Monoclonal Antibody - #BF8655

Product: NAB2 Mouse Monoclonal Antibody
Catalog: BF8655
Description: Mouse monoclonal antibody to NAB2
Application: WB
Reactivity: Human
Prediction: Mouse
Mol.Wt.: 55~70KD; 57kD(Calculated).
Uniprot: Q15742

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8655]
Specificity:
NAB2 Mouse Monoclonal Antibody detects endogenous levels of total NAB2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EGR 1 binding protein 2; EGR-1-binding protein 2; EGR1 binding protein 2; MADER; Melanoma associated delayed early response protein; Melanoma-associated delayed early response protein; MGC75085; Nab 2; nab2; NAB2_HUMAN; NGFI A binding protein 2 (EGR1 binding protein 2); NGFI A binding protein 2; NGFI-A-binding protein 2; Protein MADER;

Immunogens

Immunogen:

A synthesized peptide derived from human NAB2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q15742(NAB2_HUMAN) >>Visit HPA database.

Gene(ID):
NAB2(4665)
Expression:
Q15742 NAB2_HUMAN:

Widely expressed at low levels. Highly expressed in melanoma cell lines.

Sequence:
MHRAPSPTAEQPPGGGDSARRTLQPRLKPSARAMALPRTLGELQLYRVLQRANLLSYYETFIQQGGDDVQQLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLFSQPVPAVPVSSIPLFKISETAGTRKGSMSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSPPAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVESVERIFRSFPRGDAGEVTSLLKLNKKLARSVGHIFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQVARESTYLSSLKGSRLHPEELGGPPLKKLKQEVGEQSHPEIQQPPPGPESYVPPYRPSLEEDSASLSGESLDGHLQAVGSCPRLTPPPADLPLALPAHGLWSRHILQQTLMDEGLRLARLVSHDRVGRLSPCVPAKPPLAEFEEGLLDRCPAPGPHPALVEGRRSSVKVEAEASRQ

PTMs - Q15742 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
T8 Phosphorylation
S131 Phosphorylation
S147 Phosphorylation
S157 Phosphorylation
S159 Phosphorylation
S162 Phosphorylation
K169 Sumoylation
K169 Ubiquitination
S171 Phosphorylation
S205 Phosphorylation
S209 Phosphorylation
K269 Ubiquitination
S289 Phosphorylation
K361 Sumoylation
K361 Ubiquitination
K376 Acetylation
K376 Ubiquitination
K379 Sumoylation
T434 Phosphorylation
S471 Phosphorylation
S479 Phosphorylation
S514 Phosphorylation
S515 Phosphorylation
K517 Sumoylation

Research Backgrounds

Function:

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).

PTMs:

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.

Subcellular Location:

Nucleus.
Note: Isoform 2 is not localized to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed at low levels. Highly expressed in melanoma cell lines.

Subunit Structure:

Homomultimers may associate with EGR1 bound to DNA.

Family&Domains:

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.

The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Belongs to the NAB family.

Restrictive clause

 

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For Research Use Only.
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