ACTN3 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	ACTN3 Mouse Monoclonal Antibody
Catalog:	BF8653
Description:	Mouse monoclonal antibody to ACTN3
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat
Mol.Wt.:	103 kDa; 103kD(Calculated).
Uniprot:	Q08043

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$140 ~~280~~	In stock

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8653]

Specificity:

ACTN3 Mouse Monoclonal Antibody detects endogenous levels of total ACTN3.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Actinin alpha 3; actinin, alpha; ACTN 3; ACTN3; ACTN3_HUMAN; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 3; Alpha-Actinin 3; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-3; F actin cross linking protein; F-actin cross-linking protein; MGC117002; MGC117005; OTTHUMP00000219049; OTTHUMP00000219054;

Immunogens

Immunogen:

A synthesized peptide derived from human ACTN3, corresponding to a region within C-terminal amino acids.

Uniprot:

Q08043(ACTN3_HUMAN) >>Visit HPA database.

Gene(ID):

ACTN3(89)

Expression:

Q08043 ACTN3_HUMAN:

Expressed only in a subset of type 2 skeletal muscle fibers.

Sequence:

Q08043 ACTN3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMMVMQPEGLGAGEGRFAGGGGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRIQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFRQKASLHEAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEELARQQVNERLRRQFAAQANAIGPWIQAKVEEVGRLAAGLAGSLEEQMAGLRQQEQNIINYKTNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKQNGMMEPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTTEQVVASFKILAGDKNYITPEELRRELPAKQAEYCIRRMVPYKGSGAPAGALDYVAFSSALYGESDL

PTMs - Q08043 As Substrate

Q08043

Site	PTM Type	Source
M1	Acetylation	Uniprot
K45	Ubiquitination	Uniprot
K49	Ubiquitination	Uniprot
K61	Ubiquitination	Uniprot
T64	Phosphorylation	Uniprot
R90	Methylation	Uniprot
R93	Methylation	Uniprot
S116	Phosphorylation	Uniprot
K117	Ubiquitination	Uniprot
T137	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
T158	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
K209	Sumoylation	Uniprot
K212	Sumoylation	Uniprot
Y229	Phosphorylation	Uniprot
K250	Acetylation	Uniprot
S257	Phosphorylation	Uniprot
Y260	Phosphorylation	Uniprot
R275	Methylation	Uniprot
K278	Acetylation	Uniprot
S298	Phosphorylation	Uniprot
S319	Phosphorylation	Uniprot
S321	Phosphorylation	Uniprot
Y333	Phosphorylation	Uniprot
T354	Phosphorylation	Uniprot
T357	Phosphorylation	Uniprot
S376	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
Y436	Phosphorylation	Uniprot
S438	Phosphorylation	Uniprot
S456	Phosphorylation	Uniprot
K506	Acetylation	Uniprot
S737	Phosphorylation	Uniprot
Y851	Phosphorylation	Uniprot
Y876	Phosphorylation	Uniprot

Research Backgrounds

Q08043_ACTN3_HUMAN

Function:

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Tissue Specificity:

Expressed only in a subset of type 2 skeletal muscle fibers.

Subunit Structure:

Homodimer; antiparallel. Also forms heterodimers with ACTN2. Interacts with MYOZ1.

Family&Domains:

Belongs to the alpha-actinin family.

Research Fields

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

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AFfirm™ ACTN3 Mouse Monoclonal Antibody - #BF8653