AFfirm™ CLP1 Mouse Monoclonal Antibody - #BF8649
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
ATP/GTP binding protein; Cleavage and polyadenylation factor I subunit 1; CLP 1; clp1; CLP1 cleavage and polyadenylation factor I subunit homolog; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); CLP1, yeast, homolog of; CLP1_HUMAN; hClp1; Homolog of yeast CFIA subunit Clp1p; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Polyribonucleotide 5' hydroxyl kinase Clp1; Polyribonucleotide 5''-hydroxyl-kinase Clp1; Pre mRNA cleavage complex II protein Clp1; Pre-mRNA cleavage complex II protein Clp1;
Immunogens
- Q92989 CLP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK
PTMs - Q92989 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S30 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| T356 | Phosphorylation | Uniprot |
Research Backgrounds
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
Nucleus.
Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.
Belongs to the Clp1 family. Clp1 subfamily.
Research Fields
· Genetic Information Processing > Translation > mRNA surveillance pathway.
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