CLP1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	CLP1 Mouse Monoclonal Antibody
Catalog:	BF8649
Description:	Mouse monoclonal antibody to CLP1
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	Q92989

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$140 ~~280~~	In stock

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8649]

Specificity:

CLP1 Mouse Monoclonal Antibody detects endogenous levels of total CLP1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP/GTP binding protein; Cleavage and polyadenylation factor I subunit 1; CLP 1; clp1; CLP1 cleavage and polyadenylation factor I subunit homolog; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); CLP1, yeast, homolog of; CLP1_HUMAN; hClp1; Homolog of yeast CFIA subunit Clp1p; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Polyribonucleotide 5' hydroxyl kinase Clp1; Polyribonucleotide 5''-hydroxyl-kinase Clp1; Pre mRNA cleavage complex II protein Clp1; Pre-mRNA cleavage complex II protein Clp1;

Immunogens

Immunogen:

Uniprot:

Q92989(CLP1_HUMAN) >>Visit HPA database.

Gene(ID):

CLP1(10978)

Sequence:

Q92989 CLP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK

PTMs - Q92989 As Substrate

Q92989

Site	PTM Type	Source
S30	Phosphorylation	Uniprot
S32	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
T88	Phosphorylation	Uniprot
Y93	Phosphorylation	Uniprot
K127	Ubiquitination	Uniprot
K202	Ubiquitination	Uniprot
K267	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
S282	Phosphorylation	Uniprot
S289	Phosphorylation	Uniprot
Y313	Phosphorylation	Uniprot
K320	Ubiquitination	Uniprot
K325	Ubiquitination	Uniprot
K328	Ubiquitination	Uniprot
S344	Phosphorylation	Uniprot
K351	Ubiquitination	Uniprot
T356	Phosphorylation	Uniprot

Research Backgrounds

Q92989_CLP1_HUMAN

Function:

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54. Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.

Family&Domains:

Belongs to the Clp1 family. Clp1 subfamily.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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AFfirm™ CLP1 Mouse Monoclonal Antibody - #BF8649