RPC6 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	RPC6 Mouse Monoclonal Antibody
Catalog:	BF8648
Description:	Mouse monoclonal antibody to RPC6
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	36 kDa; 36kD(Calculated).
Uniprot:	Q9H1D9

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8648]

Specificity:

RPC6 Mouse Monoclonal Antibody detects endogenous levels of total RPC6.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA directed RNA polymerase III 39 kDa polypeptide; DNA directed RNA polymerase III 39 kDa polypeptide F; DNA directed RNA polymerase III subunit F; DNA directed RNA polymerase III subunit RPC6; DNA directed RNA polymerases III 39 kDa polypeptide; DNA-directed RNA polymerase III subunit F; DNA-directed RNA polymerase III subunit RPC6; MGC13517; Polr3f; Polymerase (RNA) III (DNA directed) polypeptide F; RNA polymerase III 39 kDa subunit; RNA polymerase III C39 subunit; RNA polymerase III subunit C6; RPC 39; RPC 6; RPC39; RPC6; RPC6_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9H1D9(RPC6_HUMAN) >>Visit HPA database.

Gene(ID):

POLR3F(10621)

Sequence:

Q9H1D9 RPC6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF

PTMs - Q9H1D9 As Substrate

Q9H1D9

Site	PTM Type	Source
A2	Acetylation	Uniprot
K7	Sumoylation	Uniprot
S59	Phosphorylation	Uniprot
S79	Phosphorylation	Uniprot
K93	Ubiquitination	Uniprot
K128	Ubiquitination	Uniprot
K140	Acetylation	Uniprot
K146	Acetylation	Uniprot
Y150	Phosphorylation	Uniprot
K191	Ubiquitination	Uniprot
K199	Ubiquitination	Uniprot
K256	Ubiquitination	Uniprot

Research Backgrounds

Q9H1D9_RPC6_HUMAN

Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA).

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex (By similarity). Directly interacts with POLR3C. Interacts with TBP and TFIIIB90 and GTF3C4 (By similarity). Interacts with MAF1.

Family&Domains:

Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Research Fields

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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