AFfirm™ COPG Mouse Monoclonal Antibody - #BF8646

Product: COPG Mouse Monoclonal Antibody
Catalog: BF8646
Description: Mouse monoclonal antibody to COPG
Application: WB
Reactivity: Human
Prediction: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 98 kDa; 98kD(Calculated).
Uniprot: Q9Y678

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 100ul $140 280 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8646]
Specificity:
COPG Mouse Monoclonal Antibody detects endogenous levels of total COPG.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Coat protein gamma cop; Coatomer protein complex, subunit gamma 1; coatomer protein complex, subunit gamma; Coatomer subunit gamma; Coatomer subunit gamma-1; COP; COPG1; COPG1_HUMAN; FLJ21068; Gamma coat protein; Gamma COP; Gamma-1-coat protein; Gamma-1-COP;

Immunogens

Immunogen:
Uniprot:

Q9Y678(COPG1_HUMAN) >>Visit HPA database.

Gene(ID):
COPG1(22820)
Sequence:
MLKKFDKKDEESGGGSNPFQHLEKSAVLQEARVFNETPINPRKCAHILTKILYLINQGEHLGTTEATEAFFAMTKLFQSNDPTLRRMCYLTIKEMSCIAEDVIIVTSSLTKDMTGKEDNYRGPAVRALCQITDSTMLQAIERYMKQAIVDKVPSVSSSALVSSLHLLKCSFDVVKRWVNEAQEAASSDNIMVQYHALGLLYHVRKNDRLAVNKMISKVTRHGLKSPFAYCMMIRVASKQLEEEDGSRDSPLFDFIESCLRNKHEMVVYEAASAIVNLPGCSAKELAPAVSVLQLFCSSPKAALRYAAVRTLNKVAMKHPSAVTACNLDLENLVTDSNRSIATLAITTLLKTGSESSIDRLMKQISSFMSEISDEFKVVVVQAISALCQKYPRKHAVLMNFLFTMLREEGGFEYKRAIVDCIISIIEENSESKETGLSHLCEFIEDCEFTVLATRILHLLGQEGPKTTNPSKYIRFIYNRVVLEHEEVRAGAVSALAKFGAQNEEMLPSILVLLKRCVMDDDNEVRDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLERALQQYTLEPSEKPFDLKSVPLATAPMAEQRTESTPITAVKQPEKVAATRQEIFQEQLAAVPEFRGLGPLFKSSPEPVALTESETEYVIRCTKHTFTNHMVFQFDCTNTLNDQTLENVTVQMEPTEAYEVLCYVPARSLPYNQPGTCYTLVALPKEDPTAVACTFSCMMKFTVKDCDPTTGETDDEGYEDEYVLEDLEVTVADHIQKVMKLNFEAAWDEVGDEFEKEETFTLSTIKTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDILVRSRLLLLDTVTMQVTARSLEELPVDIILASVG

PTMs - Q9Y678 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
S12 Phosphorylation
S16 Phosphorylation
K24 Ubiquitination
S25 Phosphorylation
T37 Phosphorylation
T91 Phosphorylation
T106 Phosphorylation
S107 Phosphorylation
S108 Phosphorylation
T110 Phosphorylation
T114 Phosphorylation
K116 Ubiquitination
Y143 Phosphorylation
K145 Methylation
K145 Ubiquitination
S162 Phosphorylation
S163 Phosphorylation
K213 Ubiquitination
T219 Phosphorylation
S237 Phosphorylation
K238 Ubiquitination
S246 Phosphorylation
K300 Ubiquitination
R304 Methylation
T310 Phosphorylation
S320 Phosphorylation
S339 Phosphorylation
S356 Phosphorylation
R359 Methylation
K465 Ubiquitination
K471 Ubiquitination
R488 Methylation
K514 Ubiquitination
K538 Ubiquitination
K540 Ubiquitination
S554 Phosphorylation
K572 Ubiquitination
K577 Ubiquitination
S578 Phosphorylation
S593 Phosphorylation
T594 Phosphorylation
T597 Phosphorylation
K600 Ubiquitination
K604 Ubiquitination
K631 Ubiquitination
T640 Phosphorylation
S642 Phosphorylation
Y646 Phosphorylation
T718 Phosphorylation
T723 Phosphorylation
S725 Phosphorylation
T742 Phosphorylation
K795 Ubiquitination
K806 Ubiquitination
K818 Ubiquitination
S872 Phosphorylation

Research Backgrounds

Function:

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).

Subcellular Location:

Cytoplasm. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>COPI-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Predominantly located in the cis-Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with ZNF289/ARFGAP2 through its C-terminal appendage domain. Interacts with EGFR upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with COPB1. Interacts with TMED10 (via C-terminus). Interacts with TMED2, TMED3, TMED7 and TMED9.

Family&Domains:

Belongs to the COPG family.

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