AWAT2 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	AWAT2 Mouse Monoclonal Antibody
Catalog:	BF8639
Description:	Mouse monoclonal antibody to AWAT2
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Rabbit, Dog
Mol.Wt.:	38 kDa; 38kD(Calculated).
Uniprot:	Q6E213

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8639]

Specificity:

AWAT2 Mouse Monoclonal Antibody detects endogenous levels of total AWAT2.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acyl CoA wax alcohol acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; Acyl-CoA wax alcohol acyltransferase 2; ARAT; Awat2; AWAT2_HUMAN; DC4; Diacylglycerol O acyltransferase 2 like 4; Diacylglycerol O acyltransferase candidate 4; Diacylglycerol O-acyltransferase 2-like protein 4; Diacylglycerol O-acyltransferase candidate 4; hDC4; hWS; Long chain alcohol O fatty acyltransferase 2; Long-chain-alcohol O-fatty-acyltransferase 2; MFAT; Multifunctional O acyltransferase; Multifunctional O-acyltransferase; Retinol O-fatty-acyltransferase; Wax synthase 2; Wax synthase; WS;

Immunogens

Immunogen:

Uniprot:

Q6E213(AWAT2_HUMAN) >>Visit HPA database.

Gene(ID):

AWAT2(158835)

Expression:

Q6E213 AWAT2_HUMAN:

Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.

Sequence:

Q6E213 AWAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRFTCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILTLGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVRMALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVTTIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII

PTMs - Q6E213 As Substrate

Q6E213

Site	PTM Type	Enzyme	Source
T176	Phosphorylation		Uniprot
S206	Phosphorylation		Uniprot

Research Backgrounds

Q6E213_AWAT2_HUMAN

Function:

Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters. Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donors and acceptors, respectively. Also possesses acyl-CoA retinol acyltransferase (ARAT) activity that catalyzes 11-cis-specific retinyl ester synthesis. Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13-cis-retinol, and all-trans-retinol substrates.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the diacylglycerol acyltransferase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ AWAT2 Mouse Monoclonal Antibody - #BF8639