ACAD9 Mouse Monoclonal Antibody | Affinity Biosciences

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ACAD9 Mouse Monoclonal Antibody - Western blot analysis of extracts from Rat spleen, using ACAD9 Mouse Monoclonal Antibody at 1/1000 dilution.

Product:	ACAD9 Mouse Monoclonal Antibody
Catalog:	BF8638
Description:	Mouse monoclonal antibody to ACAD9
Application:	WB
Reactivity:	Human, Rat
Prediction:	Mouse, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	69kDa; 69kD(Calculated).
Uniprot:	Q9H845

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Monoclonal [AFfirm8638]

Specificity:

ACAD9 Mouse Monoclonal Antibody detects endogenous levels of total ACAD9.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acyl CoA dehydrogenase 9; Acyl Coenzyme A dehydrogenase family, member 9; acyl-CoA dehydrogenase family member 9, mitochondrial; FLJ23533; MGC14452; NPD002; Very long chain acyl CoA dehydrogenase VLCAD;

Immunogens

Immunogen:

A synthesized peptide derived from human ACAD9, corresponding to a region within the internal amino acids.

Uniprot:

Q9H845(ACAD9_HUMAN) >>Visit HPA database.

Gene(ID):

ACAD9(28976)

Expression:

Q9H845 ACAD9_HUMAN:

Ubiquitously expressed in most normal human tissues and cancer cell lines with high level of expression in heart, skeletal muscles, brain, kidney and liver (PubMed:12359260). In the cerebellum uniquely expressed in the granular layer (at protein level) (PubMed:21237683).

Sequence:

Q9H845 ACAD9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGCGLFLRTTAAARACRGLVVSTANRRLLRTSPPVRAFAKELFLGKIKKKEVFPFPEVSQDELNEINQFLGPVEKFFTEEVDSRKIDQEGKIPDETLEKLKSLGLFGLQVPEEYGGLGFSNTMYSRLGEIISMDGSITVTLAAHQAIGLKGIILAGTEEQKAKYLPKLASGEHIAAFCLTEPASGSDAASIRSRATLSEDKKHYILNGSKVWITNGGLANIFTVFAKTEVVDSDGSVKDKITAFIVERDFGGVTNGKPEDKLGIRGSNTCEVHFENTKIPVENILGEVGDGFKVAMNILNSGRFSMGSVVAGLLKRLIEMTAEYACTRKQFNKRLSEFGLIQEKFALMAQKAYVMESMTYLTAGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGLGYTRDYPYERILRDTRILLIFEGTNEILRMYIALTGLQHAGRILTTRIHELKQAKVSTVMDTVGRRLRDSLGRTVDLGLTGNHGVVHPSLADSANKFEENTYCFGRTVETLLLRFGKTIMEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLRNHDHEVLLANTFCVEAYLQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRTC

Research Backgrounds

Q9H845_ACAD9_HUMAN

Function:

As part of the MCIA complex, primarily participates to the assembly of the mitochondrial complex I and therefore plays a role in oxidative phosphorylation. This moonlighting protein has also a dehydrogenase activity toward a broad range of substrates with greater specificity for long-chain unsaturated acyl-CoAs. However, in vivo, it does not seem to play a primary role in fatty acid oxidation. In addition, the function in complex I assembly is independent of the dehydrogenase activity of the protein.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.
Note: Essentially associated with membranes.

Tissue Specificity:

Ubiquitously expressed in most normal human tissues and cancer cell lines with high level of expression in heart, skeletal muscles, brain, kidney and liver. In the cerebellum uniquely expressed in the granular layer (at protein level).

Family&Domains:

Belongs to the acyl-CoA dehydrogenase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ ACAD9 Mouse Monoclonal Antibody - #BF8638