INPP5E Mouse Monoclonal Antibody | Affinity Biosciences

Product:	INPP5E Mouse Monoclonal Antibody
Catalog:	BF8637
Description:	Mouse monoclonal antibody to INPP5E
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Dog, Chicken
Mol.Wt.:	85 kDa; 70kD(Calculated).
Uniprot:	Q9NRR6

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$140 ~~280~~	In stock

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8637]

Specificity:

INPP5E Mouse Monoclonal Antibody detects endogenous levels of total INPP5E.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5-bisphosphate 5-phosphatase; 72 kDa inositol polyphosphate 5-phosphatase; Inositol polyphosphate 5 phosphatase; INP5E_HUMAN; Inpp5e; Phosphatidylinositol (4,5) bisphosphate 5 phosphatase; Phosphatidylinositol 4; Phosphatidylinositol polyphosphate 5 phosphatase type IV; Phosphatidylinositol polyphosphate 5-phosphatase type IV; PPI5PIV;

Immunogens

Immunogen:

Uniprot:

Q9NRR6(INP5E_HUMAN) >>Visit HPA database.

Gene(ID):

INPP5E(56623)

Expression:

Q9NRR6 INP5E_HUMAN:

Detected in brain, heart, pancreas, testis and spleen.

Sequence:

Q9NRR6 INP5E_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSKAENLRPSEPAPQPPEGRTLQGQLPGAPPAQRAGSPPDAPGSESPALACSTPATPSGEDPPARAAPIAPRPPARPRLERALSLDDKGWRRRRFRGSQEDLEARNGTSPSRGSVQSEGPGAPAHSCSPPCLSTSLQEIPKSRGVLSSERGSPSSGGNPLSGVASSSPNLPHRDAAVAGSSPRLPSLLPPRPPPALSLDIASDSLRTANKVDSDLADYKLRAQPLLVRAHSSLGPGRPRSPLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGADELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLYVIGVQEGCSDRREWETRLQETLGPHYVLLSSAAHGVLYMSLFIRRDLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLFITSHFTSGDGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEVFWFGDFNFRLSGGRTVVDALLCQGLVVDVPALLQHDQLIREMRKGSIFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYTDRVLYRSRHKGDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFDRELYLLGIKRRISKEIQRQQALQSQNSSTICSVS

PTMs - Q9NRR6 As Substrate

Q9NRR6

Site	PTM Type	Source
S38	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
S99	Phosphorylation	Uniprot
T109	Phosphorylation	Uniprot
S153	Phosphorylation	Uniprot
S156	Phosphorylation	Uniprot
S166	Phosphorylation	Uniprot
K220	Ubiquitination	Uniprot
S232	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot
S241	Phosphorylation	Uniprot
K254	Ubiquitination	Uniprot
S255	Phosphorylation	Uniprot
Y293	Phosphorylation	Uniprot
Y439	Phosphorylation	Uniprot
T455	Phosphorylation	Uniprot
K521	Ubiquitination	Uniprot
T542	Phosphorylation	Uniprot
K565	Ubiquitination	Uniprot

Research Backgrounds

Q9NRR6_INP5E_HUMAN

Function:

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (By similarity). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium axoneme. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle. Cytoplasm.
Note: Peripheral membrane protein associated with Golgi stacks.

Tissue Specificity:

Detected in brain, heart, pancreas, testis and spleen.

Subunit Structure:

Interacts (when prenylated) with PDE6D; this is important for normal location in cilia.

Family&Domains:

Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type IV family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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AFfirm™ INPP5E Mouse Monoclonal Antibody - #BF8637