MYLK2 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	MYLK2 Mouse Monoclonal Antibody
Catalog:	BF8635
Description:	Mouse monoclonal antibody to MYLK2
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Monkey, Chicken
Mol.Wt.:	65 kDa; 65kD(Calculated).
Uniprot:	Q9H1R3

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8635]

Specificity:

MYLK2 Mouse Monoclonal Antibody detects endogenous levels of total MYLK2.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KMLC; MLCK; MLCK, skeletal/cardiac; MLCK2; MYLK 2; MYLK2; MYLK2_HUMAN; Myosin light chain kinase 2; Myosin light chain kinase 2 skeletal muscle; Myosin light chain kinase 2 skeletal/cardiac muscle; MYOSIN LIGHT POLYPEPTIDE KINASE; Myosin light polypeptide kinase, skeletal/cardiac; Skeletal muscle myosin light chain kinase; Skeletal myosin light chain kinase; skeletal/cardiac muscle; skMLCK;

Immunogens

Immunogen:

Uniprot:

Q9H1R3(MYLK2_HUMAN) >>Visit HPA database.

Gene(ID):

MYLK2(85366)

Expression:

Q9H1R3 MYLK2_HUMAN:

Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.

Sequence:

Q9H1R3 MYLK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATENGAVELGIQNPSTDKAPKGPTGERPLAAGKDPGPPDPKKAPDPPTLKKDAKAPASEKGDGTLAQPSTSSQGPKGEGDRGGGPAEGSAGPPAALPQQTATPETSVKKPKAEQGASGSQDPGKPRVGKKAAEGQAAARRGSPAFLHSPSCPAIISSSEKLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVCTCMEKATGLKLAAKVIKKQTPKDKEMVLLEIEVMNQLNHRNLIQLYAAIETPHEIVLFMEYIEGGELFERIVDEDYHLTEVDTMVFVRQICDGILFMHKMRVLHLDLKPENILCVNTTGHLVKIIDFGLARRYNPNEKLKVNFGTPEFLSPEVVNYDQISDKTDMWSMGVITYMLLSGLSPFLGDDDTETLNNVLSGNWYFDEETFEAVSDEAKDFVSNLIVKDQRARMNAAQCLAHPWLNNLAEKAKRCNRRLKSQILLKKYLMKRRWKKNFIAVSAANRFKKISSSGALMALGV

PTMs - Q9H1R3 As Substrate

Q9H1R3

Site	PTM Type	Enzyme	Source
S143	Phosphorylation		Uniprot
S151	Phosphorylation	P49137 (MAPKAPK2)	Uniprot
K561	Ubiquitination		Uniprot
Y563	Phosphorylation		Uniprot
K584	Ubiquitination		Uniprot
S586	Phosphorylation		Uniprot
S587	Phosphorylation		Uniprot
S588	Phosphorylation		Uniprot

PTMs - Q9H1R3 As Enzyme

Q9H1R3

Substrate	Site	Source
Q06413 (MEF2C)	T80	Uniprot

Research Backgrounds

Q9H1R3_MYLK2_HUMAN

Function:

Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.

Subcellular Location:

Cytoplasm.
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.

Tissue Specificity:

Heart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.

Subunit Structure:

May interact with centrin.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ MYLK2 Mouse Monoclonal Antibody - #BF8635