TUBA4A Mouse Monoclonal Antibody | Affinity Biosciences

Product:	TUBA4A Mouse Monoclonal Antibody
Catalog:	BF8631
Description:	Mouse monoclonal antibody to TUBA4A
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat
Mol.Wt.:	50kD(Calculated).
Uniprot:	P68366

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8631]

Specificity:

TUBA4A Mouse Monoclonal Antibody detects endogenous levels of TUBA4A .

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha-tubulin 1; ALS22; B ALPHA 1; bA408E5.3; H2 ALPHA; Hum a tub1; Hum a tub2; LIS3; MGC171407; MGC55332; TBA4A_HUMAN; Testis-specific alpha-tubulin; TUBA1; TUBA1A; tuba1l; Tuba4a; Tubulin alpha 1 chain; Tubulin alpha; Tubulin alpha-1 chain; tubulin alpha-1B chain; Tubulin alpha-4A chain; Tubulin H2-alpha; Tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; Tubulin, alpha 4a; Tubulin, alpha, testis-specific; Tubulin, alpha-1;

Immunogens

Immunogen:

A synthesized peptide derived from human TUBA4A .

Uniprot:

P68366(TBA4A_HUMAN) >>Visit HPA database.

Gene(ID):

TUBA4A(7277)

Sequence:

P68366 TBA4A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE

PTMs - P68366 As Substrate

P68366

Site	PTM Type	Enzyme	Source
S6	Phosphorylation		Uniprot
Y24	Phosphorylation		Uniprot
S38	Phosphorylation		Uniprot
K40	Acetylation		Uniprot
T41	Phosphorylation		Uniprot
S48	Phosphorylation		Uniprot
C54	S-Nitrosylation		Uniprot
K60	Ubiquitination		Uniprot
T73	Phosphorylation		Uniprot
T94	Phosphorylation		Uniprot
K96	Acetylation		Uniprot
K96	Sumoylation		Uniprot
K96	Ubiquitination		Uniprot
Y103	Phosphorylation		Uniprot
Y108	Phosphorylation		Uniprot
T109	Phosphorylation		Uniprot
K112	Acetylation		Uniprot
K112	Sumoylation		Uniprot
K112	Ubiquitination		Uniprot
K124	Methylation		Uniprot
K124	Ubiquitination		Uniprot
S158	Phosphorylation		Uniprot
Y161	Phosphorylation		Uniprot
K163	Acetylation		Uniprot
K163	Methylation		Uniprot
K163	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
K166	Ubiquitination		Uniprot
Y172	Phosphorylation		Uniprot
Y185	Phosphorylation		Uniprot
S187	Phosphorylation		Uniprot
T190	Phosphorylation		Uniprot
T191	Phosphorylation		Uniprot
T193	Phosphorylation		Uniprot
T194	Phosphorylation		Uniprot
S198	Phosphorylation		Uniprot
Y210	Phosphorylation		Uniprot
T223	Phosphorylation		Uniprot
Y224	Phosphorylation		Uniprot
T225	Phosphorylation		Uniprot
S232	Phosphorylation		Uniprot
S236	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
T239	Phosphorylation		Uniprot
S241	Phosphorylation		Uniprot
T253	Phosphorylation		Uniprot
T257	Phosphorylation		Uniprot
Y262	Phosphorylation		Uniprot
T271	Phosphorylation		Uniprot
Y272	Phosphorylation		Uniprot
S277	Phosphorylation		Uniprot
K280	Acetylation		Uniprot
K280	Ubiquitination		Uniprot
Y282	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
T292	Phosphorylation		Uniprot
C295	S-Nitrosylation		Uniprot
K304	Ubiquitination		Uniprot
K311	Acetylation		Uniprot
K311	Ubiquitination		Uniprot
Y312	Phosphorylation		Uniprot
Y319	Phosphorylation		Uniprot
K326	Ubiquitination		Uniprot
K336	Sumoylation		Uniprot
K336	Ubiquitination		Uniprot
K338	Ubiquitination		Uniprot
R339	Methylation		Uniprot
S340	Phosphorylation		Uniprot
C347	S-Nitrosylation		Uniprot
T349	Phosphorylation		Uniprot
K352	Acetylation		Uniprot
K352	Sumoylation		Uniprot
K352	Ubiquitination		Uniprot
Y357	Phosphorylation		Uniprot
T361	Phosphorylation		Uniprot
K370	Acetylation		Uniprot
K370	Sumoylation		Uniprot
K370	Ubiquitination		Uniprot
C376	S-Nitrosylation		Uniprot
S379	Phosphorylation		Uniprot
K394	Acetylation		Uniprot
K394	Sumoylation		Uniprot
K394	Ubiquitination		Uniprot
Y399	Phosphorylation		Uniprot
K401	Acetylation		Uniprot
K401	Sumoylation		Uniprot
K401	Ubiquitination		Uniprot
Y408	Phosphorylation		Uniprot
S419	Phosphorylation		Uniprot
Y432	Phosphorylation	P43405 (SYK)	Uniprot
S439	Phosphorylation		Uniprot
Y440	Phosphorylation		Uniprot

Research Backgrounds

P68366_TBA4A_HUMAN

Function:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

PTMs:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.

Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Subunit Structure:

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CFAP157 (By similarity).

Family&Domains:

Belongs to the tubulin family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ TUBA4A Mouse Monoclonal Antibody - #BF8631