PPP2R5B Mouse Monoclonal Antibody | Affinity Biosciences

Product:	PPP2R5B Mouse Monoclonal Antibody
Catalog:	BF8628
Description:	Mouse monoclonal antibody to PPP2R5B
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Rat
Mol.Wt.:	57kD(Calculated).
Uniprot:	Q15173

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$140 ~~280~~	In stock

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8628]

Specificity:

PPP2R5B Mouse Monoclonal Antibody detects endogenous levels of total PPP2R5B.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2A5B_HUMAN; B56B; Beta isoform of regulatory subunit B56 protein phosphatase 2A; FLJ35411; PP2A B subunit B' beta isoform; PP2A B subunit B56 beta isoform; PP2A B subunit isoform B' beta; PP2A B subunit isoform B''-beta; PP2A B subunit isoform B56-beta; PP2A B subunit isoform PR61-beta; PP2A B subunit isoform R5-beta; PP2A B subunit PR61 beta isoform; PP2A B subunit R5 beta isoform; PPP2R5B; PR 61B; PR61B; Protein phosphatase 2 regulatory subunit B (B56) beta; Protein phosphatase 2 regulatory subunit B (B56) beta isoform; Protein phosphatase 2 regulatory subunit B' beta; Protein phosphatase 2 regulatory subunit B' beta isoform; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform;

Immunogens

Immunogen:

A synthesized peptide derived from Human PPP2R5B.

Uniprot:

Q15173(2A5B_HUMAN) >>Visit HPA database.

Gene(ID):

PPP2R5B(5526)

Expression:

Q15173 2A5B_HUMAN:

Highest expression in brain.

Sequence:

Q15173 2A5B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METKLPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQSNQQELTPLPLLKDVPASELHELLSRKLAQCGVMFDFLDCVADLKGKEVKRAALNELVECVGSTRGVLIEPVYPDIIRMISVNIFRTLPPSENPEFDPEEDEPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDSEDPREREYLKTILHRVYGKFLGLRAYIRKQCNHIFLRFIYEFEHFNGVAELLEILGSIINGFALPLKTEHKQFLVRVLIPLHSVKSLSVFHAQLAYCVVQFLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQFVKIQEPLFKQVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGTLYQVSKEHWNQTIVSLIYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKAQERQELWQGLEELRLRRLQGTQGAKEAPLQRLTPQVAASGGQS

Research Backgrounds

Q15173_2A5B_HUMAN

Function:

As the regulatory component of the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme, modulates substrate specificity, subcellular localization, and responsiveness to phosphorylation. The phosphorylated form mediates the interaction between PP2A and AKT1, leading to AKT1 dephosphorylation.

PTMs:

Ubiquitinated by E3 CUL3-KLHL15 complex; this modification leads to proteasomal degradation.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Highest expression in brain.

Subunit Structure:

Component of the serine/threonine-protein phosphatase 2A complex (PP2A). This complex consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant scaffold subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGO1. Interacts with AKT1. Interacts with CUL3 and KLHL15; this interaction leads to proteasomal degradation.

Family&Domains:

Belongs to the phosphatase 2A regulatory subunit B56 family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ PPP2R5B Mouse Monoclonal Antibody - #BF8628