AFfirm™ SLP76 Mouse Monoclonal Antibody - #BF8612

Product: SLP76 Mouse Monoclonal Antibody
Catalog: BF8612
Description: Mouse monoclonal antibody to SLP76
Application: WB
Reactivity: Human
Prediction: Mouse, Rat
Mol.Wt.: 75kD; 60kD(Calculated).
Uniprot: Q13094

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8612]
Specificity:
SLP76 Mouse Monoclonal Antibody detects endogenous levels of total SLP76.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte cytosolic protein 2; SH2 domain containing leukocyte protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein;

Immunogens

Immunogen:

A synthesized peptide derived from human SLP76.

Uniprot:

Q13094(LCP2_HUMAN) >>Visit HPA database.

Gene(ID):
LCP2(3937)
Expression:
Q13094 LCP2_HUMAN:

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

Sequence:
MALRNVPFRSEVLGWDPDSLADYFKKLNYKDCEKAVKKYHIDGARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGEDDGDYESPNEEEEAPVEDDADYEPPPSNDEEALQNSILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP

PTMs - Q13094 As Substrate

Site PTM Type Enzyme
Y29 Phosphorylation
K30 Ubiquitination
Y39 Phosphorylation
S106 Phosphorylation
S107 Phosphorylation
Y113 Phosphorylation P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK)
Y128 Phosphorylation P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK)
S130 Phosphorylation
Y145 Phosphorylation P43403 (ZAP70) , P06239 (LCK) , P42681 (TXK)
S169 Phosphorylation
S171 Phosphorylation
Y173 Phosphorylation Q08881 (ITK)
S207 Phosphorylation
S220 Phosphorylation
S222 Phosphorylation
T227 Phosphorylation
S234 Phosphorylation
S246 Phosphorylation
K260 Ubiquitination
S264 Phosphorylation
S268 Phosphorylation
R273 Methylation
S274 Phosphorylation
S298 Phosphorylation
S339 Phosphorylation
T341 Phosphorylation
S344 Phosphorylation
S374 Phosphorylation
S376 Phosphorylation Q92918 (MAP4K1)
R392 Methylation
S410 Phosphorylation
S417 Phosphorylation
Y423 Phosphorylation P06239 (LCK) , P43403 (ZAP70)
S425 Phosphorylation
Y426 Phosphorylation P06239 (LCK) , P43403 (ZAP70)
T428 Phosphorylation
K438 Ubiquitination
T454 Phosphorylation
T455 Phosphorylation
T456 Phosphorylation
Y459 Phosphorylation
Y465 Phosphorylation
Y470 Phosphorylation
Y476 Phosphorylation
Y483 Phosphorylation
K492 Ubiquitination
K516 Ubiquitination
Y522 Phosphorylation
Y532 Phosphorylation

Research Backgrounds

Function:

Involved in T-cell antigen receptor mediated signaling.

PTMs:

Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

Subunit Structure:

Interacts with SLA. Interacts with CBLB (By similarity). Interacts with GRB2. Interacts with SHB. Interacts with PRAM1. Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus). Interacts with FYB1 and the phosphorylated form of FYB2.

Family&Domains:

The SH2 domain mediates interaction with phosphorylated CD6 (PubMed:16914752). The SH2 domain mediates interaction with SHB (PubMed:12084069).

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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