SAP62 Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using SAP62 Mouse Monoclonal Antibody at 1/1000 dilution.

Western blot analysis of extracts from Rat lung, using SAP62 Mouse Monoclonal Antibody at 1/1000 dilution.

Product:	SAP62 Mouse Monoclonal Antibody
Catalog:	BF8592
Description:	Mouse monoclonal antibody to SAP62
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	65kD; 49kD(Calculated).
Uniprot:	Q15428

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8592]

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

pre-mRNA splicing factor SF3A, subunit 2; PRP11; PRPF11; SAP 62; SAP62; Sf3a2; SF3A2_HUMAN; SF3a66; spliceosome associated protein 62; Spliceosome-associated protein 62; Splicing factor 3A subunit 2; splicing factor 3a, subunit 2, 66kDa;

Immunogens

Immunogen:

A synthesized peptide derived from Human SAP62.

Uniprot:

Q15428(SF3A2_HUMAN) >>Visit HPA database.

Gene(ID):

SF3A2(8175)

Sequence:

Q15428 SF3A2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVTKQRDSEMGQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQFHFKMEKPPAPPSLPAGPPGVKRPPPPLMNGLPPRPPLPESLPPPPPGGLPLPPMPPTGPAPSGPPGPPQLPPPAPGVHPPAPVVHPPASGVHPPAPGVHPPAPGVHPPAPGVHPPTSGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPPSAGVHPQAPGVHPAAPAVHPQAPGVHPPAPGMHPQAPGVHPQPPGVHPSAPGVHPQPPGVHPSNPGVHPPTPMPPMLRPPLPSEGPGNIPPPPPTN

Research Backgrounds

Q15428_SF3A2_HUMAN

Function:

Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes, including the Bact complex. Interacts directly with the duplex formed by U2 snRNA and the intron.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62 and SF3A1/SAP114. SF3A1 functions as scaffold that interacts directly with both SF3A2 and SF3A3. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small nuclear ribonucleoprotein complex (17S U2 snRNP). Identified in the spliceosome 'E' complex, a precursor of the spliceosome 'A' complex. Identified in the spliceosome 'A' and 'B' complexes. Identified in the spliceosome 'C' complex. Interacts with HTATSF1.

Family&Domains:

Belongs to the SF3A2 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ SAP62 Mouse Monoclonal Antibody - #BF8592