HDLBP Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using HDLBP Mouse Monoclonal Antibody at 1/1000 dilution.

Western blot analysis of extracts from Rat lung, using HDLBP Mouse Monoclonal Antibody at 1/1000 dilution.

BF8531 at 1/100 staining human lung cancer and para-carcinoma tissue by IHC-P.

Product:	HDLBP Mouse Monoclonal Antibody
Catalog:	BF8531
Description:	Mouse monoclonal antibody to HDLBP
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	141 kDa; 141kD(Calculated).
Uniprot:	Q00341

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$140 ~~280~~	In stock

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8531]

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HBP; HDL binding protein; HDL-binding protein; HDLBP; high density lipoprotein binding protein; high density lipoprotein binding protein (vigilin); High density lipoprotein-binding protein; PRO2900; VGL; Vigilin; VIGLN_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q00341(VIGLN_HUMAN) >>Visit HPA database.

Gene(ID):

HDLBP(3069)

Sequence:

Q00341 VIGLN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSVAVLTQESFAEHRSGLVPQQIKVATLNSEEESDPPTYKDAFPPLPEKAACLESAQEPSGAWGNKIRPIKASVITQVFHVPLEERKYKDMNQFGEGEQAKICLEIMQRTGAHLELSLAKDQGLSIMVSGKLDAVMKARKDIVARLQTQASATVAIPKEHHRFVIGKNGEKLQDLELKTATKIQIPRPDDPSNQIKITGTKEGIEKARHEVLLISAEQDKRAVERLEVEKAFHPFIAGPYNRLVGEIMQETGTRINIPPPSVNRTEIVFTGEKEQLAQAVARIKKIYEEKKKKTTTIAVEVKKSQHKYVIGPKGNSLQEILERTGVSVEIPPSDSISETVILRGEPEKLGQALTEVYAKANSFTVSSVAAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTEDVNVAQEQIEGMVKDLINRMDYVEINIDHKFHRHLIGKSGANINRIKDQYKVSVRIPPDSEKSNLIRIEGDPQGVQQAKRELLELASRMENERTKDLIIEQRFHRTIIGQKGERIREIRDKFPEVIINFPDPAQKSDIVQLRGPKNEVEKCTKYMQKMVADLVENSYSISVPIFKQFHKNIIGKGGANIKKIREESNTKIDLPAENSNSETIIITGKRANCEAARSRILSIQKDLANIAEVEVSIPAKLHNSLIGTKGRLIRSIMEECGGVHIHFPVEGSGSDTVVIRGPSSDVEKAKKQLLHLAEEKQTKSFTVDIRAKPEYHKFLIGKGGGKIRKVRDSTGARVIFPAAEDKDQDLITIIGKEDAVREAQKELEALIQNLDNVVEDSMLVDPKHHRHFVIRRGQVLREIAEEYGGVMVSFPRSGTQSDKVTLKGAKDCVEAAKKRIQEIIEDLEAQVTLECAIPQKFHRSVMGPKGSRIQQITRDFSVQIKFPDREENAVHSTEPVVQENGDEAGEGREAKDCDPGSPRRCDIIIISGRKEKCEAAKEALEALVPVTIEVEVPFDLHRYVIGQKGSGIRKMMDEFEVNIHVPAPELQSDIIAITGLAANLDRAKAGLLERVKELQAEQEDRALRSFKLSVTVDPKYHPKIIGRKGAVITQIRLEHDVNIQFPDKDDGNQPQDQITITGYEKNTEAARDAILRIVGELEQMVSEDVPLDHRVHARIIGARGKAIRKIMDEFKVDIRFPQSGAPDPNCVTVTGLPENVEEAIDHILNLEEEYLADVVDSEALQVYMKPPAHEEAKAPSRGFVVRDAPWTASSSEKAPDMSSSEEFPSFGAQVAPKTLPWGPKR

PTMs - Q00341 As Substrate

Q00341

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
T8	Phosphorylation	O14757 (CHEK1)	Uniprot
S11	Phosphorylation		Uniprot
K25	Ubiquitination		Uniprot
T28	Phosphorylation		Uniprot
S31	Phosphorylation		Uniprot
S35	Phosphorylation		Uniprot
T39	Phosphorylation		Uniprot
Y40	Phosphorylation		Uniprot
K41	Ubiquitination		Uniprot
K90	Ubiquitination		Uniprot
T111	Phosphorylation		Uniprot
S126	Phosphorylation		Uniprot
K138	Ubiquitination		Uniprot
K159	Ubiquitination		Uniprot
K172	Ubiquitination		Uniprot
K179	Ubiquitination		Uniprot
K197	Ubiquitination		Uniprot
K202	Ubiquitination		Uniprot
S216	Phosphorylation		Uniprot
K221	Ubiquitination		Uniprot
K231	Ubiquitination		Uniprot
Y241	Phosphorylation		Uniprot
T271	Phosphorylation		Uniprot
K274	Ubiquitination		Uniprot
K294	Ubiquitination		Uniprot
T296	Phosphorylation		Uniprot
T297	Phosphorylation		Uniprot
K308	Acetylation		Uniprot
K308	Ubiquitination		Uniprot
K314	Ubiquitination		Uniprot
S317	Phosphorylation		Uniprot
K349	Ubiquitination		Uniprot
Y358	Phosphorylation		Uniprot
K360	Ubiquitination		Uniprot
S363	Phosphorylation		Uniprot
S368	Phosphorylation		Uniprot
K383	Ubiquitination		Uniprot
K389	Ubiquitination		Uniprot
T402	Phosphorylation		Uniprot
Y437	Phosphorylation		Uniprot
K453	Ubiquitination		Uniprot
S454	Phosphorylation		Uniprot
Y465	Phosphorylation		Uniprot
K466	Ubiquitination		Uniprot
S468	Phosphorylation		Uniprot
K477	Ubiquitination		Uniprot
K494	Ubiquitination		Uniprot
K510	Ubiquitination		Uniprot
K526	Ubiquitination		Uniprot
K536	Acetylation		Uniprot
K536	Ubiquitination		Uniprot
K550	Ubiquitination		Uniprot
S581	Phosphorylation		Uniprot
Y582	Phosphorylation		Uniprot
S583	Phosphorylation		Uniprot
K594	Ubiquitination		Uniprot
K599	Ubiquitination		Uniprot
K605	Ubiquitination		Uniprot
S611	Phosphorylation		Uniprot
T613	Phosphorylation		Uniprot
K614	Ubiquitination		Uniprot
S624	Phosphorylation		Uniprot
K632	Methylation		Uniprot
K632	Ubiquitination		Uniprot
S645	Phosphorylation		Uniprot
S659	Phosphorylation		Uniprot
K663	Ubiquitination		Uniprot
S667	Phosphorylation		Uniprot
T671	Phosphorylation		Uniprot
K672	Ubiquitination		Uniprot
K711	Ubiquitination		Uniprot
K713	Ubiquitination		Uniprot
K714	Ubiquitination		Uniprot
K723	Ubiquitination		Uniprot
S727	Phosphorylation		Uniprot
K749	Ubiquitination		Uniprot
S756	Phosphorylation		Uniprot
K769	Ubiquitination		Uniprot
K779	Ubiquitination		Uniprot
K810	Ubiquitination		Uniprot
S840	Phosphorylation		Uniprot
S844	Phosphorylation		Uniprot
K846	Ubiquitination		Uniprot
K883	Ubiquitination		Uniprot
K892	Methylation		Uniprot
S894	Phosphorylation		Uniprot
S904	Phosphorylation		Uniprot
C940	S-Nitrosylation		Uniprot
S944	Phosphorylation		Uniprot
K991	Ubiquitination		Uniprot
S993	Phosphorylation		Uniprot
K1031	Ubiquitination		Uniprot
K1039	Ubiquitination		Uniprot
R1048	Methylation		Uniprot
K1054	Ubiquitination		Uniprot
S1056	Phosphorylation		Uniprot
T1058	Phosphorylation		Uniprot
K1062	Ubiquitination		Uniprot
K1066	Ubiquitination		Uniprot
T1076	Phosphorylation		Uniprot
K1108	Ubiquitination		Uniprot
Y1210	Phosphorylation		Uniprot
S1223	Phosphorylation		Uniprot
R1224	Methylation		Uniprot
S1236	Phosphorylation		Uniprot
S1237	Phosphorylation		Uniprot
K1240	Ubiquitination		Uniprot
S1247	Phosphorylation		Uniprot
S1252	Phosphorylation		Uniprot
T1261	Phosphorylation		Uniprot

Research Backgrounds

Q00341_VIGLN_HUMAN

Function:

Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.

Subcellular Location:

Cytoplasm. Nucleus.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ HDLBP Mouse Monoclonal Antibody - #BF8531