AFfirm™ Nestin Mouse Monoclonal Antibody - #BF8504

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Product: Nestin Mouse Monoclonal Antibody
Catalog: BF8504
Description: Mouse monoclonal antibody to Nestin
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 160kD,210kD; 177kD(Calculated).
Uniprot: P48681

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Monoclonal [AFfirm8504]
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin;

Immunogens

Immunogen:
Uniprot:

P48681(NEST_HUMAN) >>Visit HPA database.

Gene(ID):
NES(10763)
Expression:
P48681 NEST_HUMAN:

CNS stem cells.

Sequence:
MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDELAALRALVDQRWREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEKCARAWLSSQVAELERELEALRVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQARERLGRAVQGAREGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQFPRTPEGRRLGSLLPVLSPTSLPSPLPATLETPVPAFLKNQEFLQARTPTLASTPIPPTPQAPSPAVDAEIRAQDAPLSLLQTQGGRKQAPEPLRAEARVAIPASVLPGPEEPGGQRQEASTGQSPEDHASLAPPLSPDHSSLEAKDGESGGSRVFSICRGEGEGQIWGLVEKETAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVPLEKETLKSLGEEIQESLKTLENQSHETLERENQECPRSLEEDLETLKSLEKENKELLKDVEVVRPLEKEAVGQLKPTGKEDTQTLQSLQKENQELMKSLEGNLETFLFPGTENQELVSSLQENLESLTALEKENQEPLRSPEVGDEEALRPLTKENQEPLRSLEDENKEAFRSLEKENQEPLKTLEEEDQSIVRPLETENHKSLRSLEEQDQETLRTLEKETQQRRRSLGEQDQMTLRPPEKVDLEPLKSLDQEIARPLENENQEFLKSLKEESVEAVKSLETEILESLKSAGQENLETLKSPETQAPLWTPEEINQGAMNPLEKEIQEPLESVEVNQETFRLLEEENQESLRSLGAWNLENLRSPEEVDKESQRNLEEEENLGKGEYQESLRSLEEEGQELPQSADVQRWEDTVEKDQELAQESPPGMAGVENEDEAELNLREQDGFTGKEEVVEQGELNATEEVWIPGEGHPESPEPKEQRGLVEGASVKGGAEGLQDPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPAMGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEGPRKDLEEAGGLGTEFSELPGKSRDPWEPPREGREESEAEAPRGAEEAFPAETLGHTGSDAPSPWPLGSEEAEEDVPPVLVSPSPTYTPILEDAPGPQPQAEGSQEASWGVQGRAEALGKVESEQEELGSGEIPEGPQEEGEESREESEEDELGETLPDSTPLGFYLRSPTSPRWDPTGEQRPPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED

PTMs - P48681 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y22 Phosphorylation
K27 Ubiquitination
S37 Phosphorylation
K74 Ubiquitination
K120 Ubiquitination
K259 Ubiquitination
K271 Ubiquitination
S276 Phosphorylation
S294 Phosphorylation
S296 Phosphorylation
T304 Phosphorylation
S311 Phosphorylation
T315 Phosphorylation Q00535 (CDK5) , P06493 (CDK1)
S320 Phosphorylation
K321 Ubiquitination
T322 Phosphorylation
S323 Phosphorylation
S325 Phosphorylation
K330 Ubiquitination
T338 Phosphorylation
S346 Phosphorylation
S352 Phosphorylation
T354 Phosphorylation
S355 Phosphorylation
S358 Phosphorylation
T363 Phosphorylation
T366 Phosphorylation
T382 Phosphorylation
T384 Phosphorylation
S387 Phosphorylation
T388 Phosphorylation
T393 Phosphorylation
S398 Phosphorylation
S413 Phosphorylation
T417 Phosphorylation
S455 Phosphorylation
T456 Phosphorylation
S459 Phosphorylation
S465 Phosphorylation
S471 Phosphorylation
S475 Phosphorylation
S476 Phosphorylation
S484 Phosphorylation
S487 Phosphorylation
S491 Phosphorylation
K507 Ubiquitination
K514 Ubiquitination
S518 Phosphorylation
K532 Ubiquitination
S537 Phosphorylation
T545 Phosphorylation
S548 Phosphorylation
S556 Phosphorylation
S564 Phosphorylation
T567 Phosphorylation
S578 Phosphorylation
T585 Phosphorylation
K587 Ubiquitination
S588 Phosphorylation
S627 Phosphorylation
S638 Phosphorylation
S680 Phosphorylation
T693 Phosphorylation
S702 Phosphorylation
K708 Ubiquitination
S713 Phosphorylation
K716 Ubiquitination
S731 Phosphorylation
S743 Phosphorylation
S746 Phosphorylation
T757 Phosphorylation
S768 Phosphorylation
T776 Phosphorylation
S790 Phosphorylation
K808 Ubiquitination
S809 Phosphorylation
K811 Sumoylation
S814 Phosphorylation
K819 Ubiquitination
S820 Phosphorylation
T823 Phosphorylation
S828 Phosphorylation
K830 Ubiquitination
S831 Phosphorylation
T839 Phosphorylation
S842 Phosphorylation
T845 Phosphorylation
T851 Phosphorylation
S891 Phosphorylation
S894 Phosphorylation
S905 Phosphorylation
K911 Ubiquitination
S913 Phosphorylation
K925 Ubiquitination
Y928 Phosphorylation
S931 Phosphorylation
S934 Phosphorylation
S945 Phosphorylation
S965 Phosphorylation
S1016 Phosphorylation
S1030 Phosphorylation
S1128 Phosphorylation
S1175 Phosphorylation
Y1225 Phosphorylation
S1261 Phosphorylation
S1282 Phosphorylation
S1286 Phosphorylation
T1299 Phosphorylation Q00535 (CDK5)
S1307 Phosphorylation
S1310 Phosphorylation
S1347 Phosphorylation
S1409 Phosphorylation
S1418 Phosphorylation
S1441 Phosphorylation
S1442 Phosphorylation
S1445 Phosphorylation
S1450 Phosphorylation
S1451 Phosphorylation
S1452 Phosphorylation
S1464 Phosphorylation
S1472 Phosphorylation
S1489 Phosphorylation
S1492 Phosphorylation
S1496 Phosphorylation
S1498 Phosphorylation
S1502 Phosphorylation
S1521 Phosphorylation
S1571 Phosphorylation
S1577 Phosphorylation
S1585 Phosphorylation
T1589 Phosphorylation
S1590 Phosphorylation
K1605 Ubiquitination
S1615 Phosphorylation
S1617 Phosphorylation
S1618 Phosphorylation

Research Backgrounds

Function:

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

PTMs:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

Tissue Specificity:

CNS stem cells.

Subunit Structure:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity).

Family&Domains:

Belongs to the intermediate filament family.

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