AFfirm™ ORC3 Mouse Monoclonal Antibody - #BF8479

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Product: ORC3 Mouse Monoclonal Antibody
Catalog: BF8479
Description: Mouse monoclonal antibody to ORC3
Application: WB
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 82kDa,73kDa; 82kD(Calculated).
Uniprot: Q9UBD5

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Monoclonal [AFfirm8479]
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Homolog of Latheo; homolog of latheo, Drosophila; LAT; LATHEO; LATHEO, drosophila, homolog of; orc3; ORC3_HUMAN; ORC3L; Origin recognition complex subunit 3; Origin recognition complex subunit 3 honolog; Origin Recognition Complex Subunit 3 Isoform 1; Origin recognition complex subunit 3 like; Origin recognition complex subunit Latheo; Origin Recognition Complex, subunit 3, S. cerevisiae, homolog of; Origin recognition complex, subunit 3-like (S. cerevisiae); Origin recognition complex, subunit 3-like (yeast);

Immunogens

Immunogen:
Uniprot:

Q9UBD5(ORC3_HUMAN) >>Visit HPA database.

Gene(ID):
ORC3(23595)
Sequence:
MATSSMSKGCFVFKPNSKKRKISLPIEDYFNKGKNEPEDSKLRFETYQLIWQQMKSENERLQEELNKNLFDNLIEFLQKSHSGFQKNSRDLGGQIKLREIPTAALVLGVNVTDHDLTFGSLTEALQNNVTPYVVSLQAKDCPDMKHFLQKLISQLMDCCVDIKSKEEESVHVTQRKTHYSMDSLSSWYMTVTQKTDPKMLSKKRTTSSQWQSPPVVVILKDMESFATKVLQDFIIISSQHLHEFPLILIFGIATSPIIIHRLLPHAVSSLLCIELFQSLSCKEHLTTVLDKLLLTTQFPFKINEKVLQVLTNIFLYHDFSVQNFIKGLQLSLLEHFYSQPLSVLCCNLPEAKRRINFLSNNQCENIRRLPSFRRYVEKQASEKQVALLTNERYLKEETQLLLENLHVYHMNYFLVLRCLHKFTSSLPKYPLGRQIRELYCTCLEKNIWDSEEYASVLQLLRMLAKDELMTILEKCFKVFKSYCENHLGSTAKRIEEFLAQFQSLDETKEEEDASGSQPKGLQKTDLYHLQKSLLEMKELRRSKKQTKFEVLRENVVNFIDCLVREYLLPPETQPLHEVVYFSAAHALREHLNAAPRIALHTALNNPYYYLKNEALKSEEGCIPNIAPDICIAYKLHLECSRLINLVDWSEAFATVVTAAEKMDANSATSEEMNEIIHARFIRAVSELELLGFIKPTKQKTDHVARLTWGGC

PTMs - Q9UBD5 As Substrate

Site PTM Type Enzyme
K21 Acetylation
S23 Phosphorylation
Y29 Phosphorylation
K32 Ubiquitination
K34 Acetylation
K41 Acetylation
K41 Ubiquitination
T46 Phosphorylation
Y47 Phosphorylation
K55 Ubiquitination
S56 Phosphorylation
K86 Ubiquitination
K96 Ubiquitination
K145 Acetylation
K163 Ubiquitination
K165 Ubiquitination
K198 Ubiquitination
S207 Phosphorylation
S208 Phosphorylation
Y375 Phosphorylation
K383 Ubiquitination
K428 Ubiquitination
K480 Ubiquitination
K492 Ubiquitination
K508 Ubiquitination
S514 Phosphorylation
S516 Phosphorylation
K523 Ubiquitination
Y527 Phosphorylation
K531 Ubiquitination
S532 Phosphorylation
K537 Ubiquitination
K543 Acetylation
Y607 Phosphorylation
K611 Ubiquitination
K616 Ubiquitination
S685 Phosphorylation
K694 Ubiquitination

Research Backgrounds

Function:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.

PTMs:

Multi-mono-ubiquitinated by OBI1; ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.

Family&Domains:

Belongs to the ORC3 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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