AFfirm™ CAMKK1 Mouse Monoclonal Antibody - #BF8465

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Product: CAMKK1 Mouse Monoclonal Antibody
Catalog: BF8465
Description: Mouse monoclonal antibody to CAMKK1
Application: WB IHC
Reactivity: Human, Rat
Mol.Wt.: 56 kDa; 56kD(Calculated).
Uniprot: Q8N5S9

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Monoclonal [AFfirm8465]
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ca2+/calmodulin-dependent protein kinase kinase alpha; Calcium/calmodulin dependent protein kinase kinase; Calcium/calmodulin dependent protein kinase kinase 1 alpha; Calcium/calmodulin dependent protein kinase kinase 1; Calcium/calmodulin dependent protein kinase kinase alpha; Calcium/calmodulin-dependent protein kinase kinase 1; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CaM-KK alpha; CaMKK 1; CaMKK alpha; CAMKK alpha protein; CaMKK; Camkk1; CAMKKA; DKFZp761M0423; KKCC1_HUMAN; MGC34095; OTTMUSP00000006419;

Immunogens

Immunogen:
Uniprot:

Q8N5S9(KKCC1_HUMAN) >>Visit HPA database.

Gene(ID):
CAMKK1(84254)
Sequence:
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLVKEGFGEGGKSPELPGVQEDEAAS

PTMs - Q8N5S9 As Substrate

Site PTM Type Enzyme
Phosphorylation
S52 Phosphorylation
S67 Phosphorylation
S69 Phosphorylation
K72 Ubiquitination
S74 Phosphorylation P17612 (PRKACA)
S82 Phosphorylation
Y83 Phosphorylation
T93 Phosphorylation
S97 Phosphorylation
S100 Phosphorylation
R102 Methylation
T108 Phosphorylation
K277 Ubiquitination
S443 Phosphorylation
T446 Phosphorylation
S452 Phosphorylation
S458 Phosphorylation
R469 Methylation
R472 Methylation
S473 Phosphorylation
S475 Phosphorylation P17612 (PRKACA)
S492 Phosphorylation

PTMs - Q8N5S9 As Enzyme

Substrate Site Source
P31749-1 (AKT1) T308 Uniprot
Q13131 (PRKAA1) T183 Uniprot
Q14012 (CAMK1) T177 Uniprot
Q16566 (CAMK4) S12 Uniprot
Q16566 (CAMK4) S13 Uniprot
Q16566 (CAMK4) T200 Uniprot
Q8IU85 (CAMK1D) T180 Uniprot
Q8TDC3 (BRSK1) T189 Uniprot
Q8TDC3-2 (BRSK1) T205 Uniprot

Research Backgrounds

Function:

Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.

PTMs:

Appears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4 (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CAMK4 and calmodulin.

Family&Domains:

The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.

The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

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