HtrA2 Mouse Monoclonal Antibody | Affinity Biosciences

IF/ICC

1/1

Product:	HtrA2 Mouse Monoclonal Antibody
Catalog:	BF8227
Description:	Mouse monoclonal antibody to HtrA2
Application:	IF/ICC
Reactivity:	Human
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	O43464

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8227]

Specificity:

HtrA2 Mouse Monoclonal Antibody detects endogenous levels of total HtrA2.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA, E. coli, homolog of, 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Protease serine 25; PRSS 25; PRSS25; Serine protease 25; Serine protease HTRA2; Serine protease HTRA2 mitochondrial; Serine protease htra2 mitochondrial precursor; Serine protease omi; Serine proteinase OMI;

Immunogens

Immunogen:

Uniprot:

O43464(HTRA2_HUMAN) >>Visit HPA database.

Gene(ID):

HTRA2(27429)

Expression:

O43464 HTRA2_HUMAN:

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Sequence:

O43464 HTRA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

PTMs - O43464 As Substrate

O43464

Site	PTM Type	Enzyme	Source
R5	Methylation		Uniprot
R8	Methylation		Uniprot
T51	Phosphorylation		Uniprot
T54	Phosphorylation		Uniprot
S96	Phosphorylation		Uniprot
S142	Phosphorylation	Q9BXM7 (PINK1) , P53778 (MAPK12)	Uniprot
T157	Phosphorylation		Uniprot
S212	Phosphorylation	P31751 (AKT2) , P31749 (AKT1)	Uniprot
K237	Ubiquitination		Uniprot
T242	Phosphorylation		Uniprot
T326	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
K395	Ubiquitination		Uniprot
S400	Phosphorylation	Q00535 (CDK5)	Uniprot
T453	Phosphorylation		Uniprot

Research Backgrounds

O43464_HTRA2_HUMAN

Function:

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

PTMs:

Autoproteolytically activated.

Subcellular Location:

Mitochondrion intermembrane space. Mitochondrion membrane>Single-pass membrane protein.
Note: Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.

Tissue Specificity:

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Subunit Structure:

Homotrimer. Interacts with MXI2. Interacts with THAP5 under apoptotic conditions. The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with BIRC6/bruce.

Family&Domains:

The mature N-terminus is involved in the interaction with XIAP.

The PDZ domain mediates interaction with MXI2.

Belongs to the peptidase S1C family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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