AFfirm™ n-Myc Mouse Monoclonal Antibody - #BF8420
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
bHLHe37; Class E basic helix-loop-helix protein 37; MODED; mycn; MYCN_HUMAN; N myc; N myc proto oncogene protein; N-myc proto-oncogene protein; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene; Neuroblastoma MYC oncogene; NMYC; NMYC proto oncogene protein; ODED; Oncogene NMYC; pp65/67; V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived; v myc myelocytomatosis viral related oncogene neuroblastoma derived;
Immunogens
A synthesized peptide derived from human n-Myc.
Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).
- P04198 MYCN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC
PTMs - P04198 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S64 | Phosphorylation | Uniprot | |
| R65 | Methylation | Uniprot | |
| K133 | Acetylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| R238 | Methylation | Uniprot | |
| R242 | Methylation | Uniprot | |
| S261 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| S263 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) | Uniprot |
| K321 | Ubiquitination | Uniprot | |
| S355 | Phosphorylation | Uniprot |
Research Backgrounds
Positively regulates the transcription of MYCNOS in neuroblastoma cells.
Phosphorylated by GSK3-beta which may promote its degradation. Phosphorylated by AURKA.
Nucleus.
Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1. Interacts with MYCNOS. Interacts with AURKA; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to unphosphorylated MYCN. Interacts with FBXW7; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN.
Research Fields
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
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