DKK1 Mouse Monoclonal Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/5

Western blot analysis of extracts from various samples, using DKK1 Mouse Monoclonal Antibody at 1/1000 dilution.

BF8416 at 1/100 staining human pancreatic cancer by IHC-P.

BF8416 staining Hela cells by IF/ICC.

Product:	DKK1 Mouse Monoclonal Antibody
Catalog:	BF8416
Description:	Mouse monoclonal antibody to DKK1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Xenopus
Mol.Wt.:	28-40kDa; 29kD(Calculated).
Uniprot:	O94907

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8416]

Specificity:

DKK1 Mouse Monoclonal Antibody detects endogenous levels of total DKK1

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dickkopf 1; Dickkopf 1 homolog; Dickkopf 1 like; Dickkopf homolog 1; Dickkopf like protein 1; Dickkopf related protein 1; Dickkopf WNT signaling pathway inhibitor 1; Dickkopf-1; Dickkopf-related protein 1; DKK 1; Dkk-1; Dkk1; DKK1_HUMAN; hDkk 1; hDkk-1; SK;

Immunogens

Immunogen:

Uniprot:

O94907(DKK1_HUMAN) >>Visit HPA database.

Gene(ID):

DKK1(22943)

Expression:

O94907 DKK1_HUMAN:

Placenta.

Sequence:

O94907 DKK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMALGAAGATRVFVAMVAAALGGHPLLGVSATLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH

PTMs - O94907 As Substrate

O94907

Site	PTM Type	Source
S61	O-Glycosylation	Uniprot
Y132	Phosphorylation	Uniprot
S140	Phosphorylation	Uniprot
S141	Phosphorylation	Uniprot
T155	O-Glycosylation	Uniprot
S163	O-Glycosylation	Uniprot
T164	O-Glycosylation	Uniprot
S169	O-Glycosylation	Uniprot
T172	O-Glycosylation	Uniprot
T173	O-Glycosylation	Uniprot
T181	O-Glycosylation	Uniprot
T221	Phosphorylation	Uniprot
S255	Phosphorylation	Uniprot
N256	N-Glycosylation	Uniprot
S257	Phosphorylation	Uniprot
S258	Phosphorylation	Uniprot
T262	Phosphorylation	Uniprot

Research Backgrounds

O94907_DKK1_HUMAN

Function:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).

Subcellular Location:

Secreted.

Tissue Specificity:

Placenta.

Subunit Structure:

Interacts with LRP6. Interacts (via the C-termianl Cys-rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt-mediated signaling. Forms a ternary complex with LRP6 and KREM1. Interacts with KREM1.

Family&Domains:

The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

Belongs to the dickkopf family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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