AFfirm™ Paxillin Mouse Monoclonal Antibody - #BF8414
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
FLJ16691; FLJ23042; Paired box protein Pax 1; PAX 1; PAX1; PAXI_HUMAN; Paxillin alpha; Paxillin; PXN; PXN protein;
Immunogens
A synthesized peptide derived from human Paxillin.
- P49023 PAXI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC
PTMs - P49023 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | A0A059VC25 (FAK) , Q13882 (PTK6) , P00519 (ABL1) , Q05397 (PTK2) | Uniprot |
| S32 | Phosphorylation | Uniprot | |
| Y33 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | P12931 (SRC) | Uniprot |
| S83 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Q16539 (MAPK14) , Q00535 (CDK5) | Uniprot |
| Y88 | Phosphorylation | P12931 (SRC) | Uniprot |
| S90 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| C108 | S-Nitrosylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | P00519 (ABL1) , Q05397 (PTK2) , Q13882 (PTK6) , A0A059VC25 (FAK) | Uniprot |
| S119 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| S126 | Phosphorylation | Q9UBE8 (NLK) | Uniprot |
| S130 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T132 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| Y181 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| S260 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Q13177 (PAK2) , O96013 (PAK4) , Q13153 (PAK1) | Uniprot |
| S274 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| K277 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot |
Research Backgrounds
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility.
Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion. Cytoplasm>Cell cortex.
Note: Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389).
Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Interacts with SORBS1, PARVA and PARVB. Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent. Interacts with LIMA1; this complex stabilizes actin dynamics.
Belongs to the paxillin family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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